文献类型：会议论文

英文题名：Inhibition Performance of Lignocellulose Degradation Products on Industrial Cellulase Enzymes During Cellulose Hydrolysis

作者：Jing, Xinyun[1];Zhang, Xiaoxi[2];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jilin Fuel Alcohol Co Ltd, PetroChina Corp, Jilin Econ Dev Zone, Changchun 132101, Jilin, Peoples R China

会议论文集：12th Asia-Pacific-Confederation-of-Chemical-Engineering (APCChE 2008)

会议日期：AUG 04-06, 2008

会议地点：Dalian, PEOPLES R CHINA

语种：英文

外文关键词：Inhibition; Lignocellulose degradation products; Cellulase enzyme; Hydrolysis; Apparent inhibition parameters

摘要：This study examined the inhibition performance by the major lignocellulose degradation products, including organic acids, furan derivatives, lignin derivatives, and ethanol, on a broadly used commercial cellulase enzyme Spezyme CP (Genencor International, Rochester, NY, USA) to cellulose hydrolysis at both the well-mixing state (shaking flask) and the static state (test tube). The cellulase activity in the cellulase complex of Spezyme CP was assumed to be one single "cellulase", and the apparent kinetic parameters of this cellulase enzyme were measured as an approximate index of the inhibitory effect to the industrial cellulase enzyme. The inhibition performance of these degradation products was compared and analyzed using the determined apparent kinetic parameters. All the degradation products strongly inhibit the cellulose hydrolysis by cellulase enzyme, and the inhibitions on cellulase were all competitive type. The order of the inhibition strength by the lignocellulose degradation products to cellulase is lignin derivatives > furan derivatives > organic acids > ethanol. This study gave a quantitative view to the enzymatic hydrolysis of lignocellulose under the inhibition performance of the lignocellulose degradation products and will help to understand the lignocellulose recalcitrance to enzyme hydrolysis.