文献类型：期刊文献

英文题名：Salt stress induced lipid accumulation in heterotrophic culture cells of Chlorella protothecoides: Mechanisms based on the multi-level analysis of oxidative response, key enzyme activity and biochemical alteration

作者：Wang, Tao[1];Ge, Haiyan[1];Liu, Tingting[1];Tian, Xiwei[1];Wang, Zejian[1];Guo, Meijin[1];Chu, Ju[1];Zhuang, Yingping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：228

起止页码：18

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20161802330401);WOS:【SCI-EXPANDED(收录号:WOS:000377786500005)】；

基金：This work was supported by the National Basic Research Program of China (973 Project) (Grant Nos. 2013CB733600, 2011CB200904 and 2012CB72100X), and National High Technology Research and Development Program (863 Project) (2015AA021005).

语种：英文

外文关键词：Chlorella protothecoides; Heterotrophic culture; Lipid; Microalgae; ROS; Salt stress

摘要：Salt stress as an effective stress factor that could improve the lipid content and lipid yield of glucose in the heterotrophic culture cells of Chlorella protothecoides was demonstrated in this study. The highest lipid content of 41.2% and lipid yield of 185.8 mg/g were obtained when C. protothecoides was stressed under 30 g/L NaCl condition at its late logarithmic growth phase. Moreover, the effects of salt and osmotic stress on lipid accumulation were comparatively analyzed, and it was found that the effects of NaCl and KCl stress had no significant differences at the same osmolarity level of 1150 mOsm/kg with lipid contents of 41.7 and 40.8% as well as lipid yields of 192.9 and 186.8 mg/g, respectively, whereas these results were obviously higher than those obtained under the iso-osmotic glycerol and sorbitol stresses. Furthermore, basing on the multi-level analysis of oxidative response, key enzyme activity and biochemical alteration, the superior performance of salt stress driving lipid over-synthesis was probably ascribed to the more ROS production as a result of additional ion effect besides the osmotic effect, subsequently mediating the alteration from carbohydrate storage to lipid accumulation in signal transduction process of C. protothecoides. (C) 2016 Published by Elsevier B.V.