文献类型：期刊文献

英文题名：Combination of normal-phase medium-pressure liquid chromatography and high-performance counter-current chromatography for preparation of ginsenoside-ro from panax ginseng with high recovery and efficiency

作者：Cheng, Yijun[1,2]; Liang, Qionglin[2]; Hu, Ping[3]; Wang, Yiming[2]; Jun, Frank Wu[4]; Luo, Guoan[1,2]

机构：[1] Modern Engineering Center for Traditional Chinese Medicine, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, China; [3] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; [4] XiangCam TCM Research Centre Ltd, Unit 16, Babraham Research Campus, Meditrina, Cambridge CB22 3AT, United Kingdom

年份：2010

卷号：73

期号：1

起止页码：397

外文期刊名：Separation and Purification Technology

收录：EI(收录号：20135017085422)

语种：英文

外文关键词：Recovery - High performance liquid chromatography - Drug products - Electrospray ionization - High pressure liquid chromatography - Mass spectrometry - Efficiency - Metabolites

摘要：Ginsenoside-Ro which belongs to the oleanane-type saponins has anti-inflammatory, anti-platelet, anticomplementary and immunomodulatory activities. The present paper described the preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency by combination of normalphase medium-pressure liquid chromatography (NP-MPLC) and high-performance counter-current chromatography (HPCCC). The crude sample was preliminarily chromatographed by NP-MPLC to enrich ginsenoside-Ro to the purity of 70.2% with 95.0% recovery. Then, the enriched sample was further purified by HPCCC with a solvent system composed of ethyl acetate-isopropanol-0.1% formic acid (3:1:5, v/v), where 61mg ginsenoside-Ro was obtained from 100mg enriched sample with 96.0% purity and 83.4% recovery. The overall recovery of ginsenoside-Ro was 79.2%, whose structure was identified by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) finally. ? 2010 Elsevier B.V.