文献类型：期刊文献

中文题名：豹蛙核酸酶与斑蝥酸钠对肺腺癌细胞增殖的协同抑制作用

英文题名：Synergistic Inhibition Effect of Onconase and Sodium Cantharidate on the Growth of Human Lung Adenocarcinoma Cells

作者：翟昌宝[1];沈如凌[2];胡晓珺[1];徐殿胜[1];费俭[2,3];王庆诚[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海南方模式生物研究中心,上海201203;[3]同济大学贵州神奇民族医药研究中心,上海200092

年份：2013

卷号：35

期号：3

起止页码：328

中文期刊名：中国细胞生物学学报

外文期刊名：Chinese Journal of Cell Biology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

语种：中文

中文关键词：协同抑制作用;豹蛙核酸酶;斑蝥酸钠;肺腺癌;SPC-A-1;A549

外文关键词：synergistic inhibitory effect; Onconase; sodium cantharidate; lung adenocarcinoma; SPC-A-1; A549

摘要：豹蛙核酸酶(onconase,Onc)是从美洲北方豹蛙卵母细胞中提取的一种核糖核酸酶,对许多肿瘤细胞都具有杀伤作用。斑蝥素(cantharidin)是存在于芫青科昆虫斑蝥体内的一种天然防御性毒素,斑蝥酸钠(sodium cantharidate,SCA)是斑蝥素半合成衍生物。鉴于Onc与SCA对非小细胞肺癌都具有杀伤作用,采用MTT法测定Onc与SCA单独与联合作用于两株肺腺癌细胞的IC50值,运用联合作用指数(combination index,CI)和等效线分析评价两者联合作用的效果。结果表明,Onc与SCA联合作用时,CI值均小于0.7,等效线分析图显示,代表Onc与SCA联合作用的点均位于加成线下方,Onc与SCA对肺腺癌SPC-A-1、A549细胞株增殖的抑制作用具有协同效应。用流式细胞仪进行的凋亡细胞检测结果也支持上述"Onc/SCA联合使用具有协同抗癌作用"的结论。
Onconase （Onc） is a ribonuclease purified from oocytes of Ranapipiens, which possesses potent anti- tumor activity. Cantharidin is a natural defensive toxin, existing in Mylabrisphalerata pallas or Mylabriscichorii linnaeus. Sodium cantharidate is a semi-synthetic derivative of cantharidin. The cytotoxicity of Onconase and sodium cantharidate alone or combined on the growth of SPC-A-1 and A549 non-small cell lung cancer （NSCLC） cell lines was measured by MTT method. The anti-tumor joint effect of Onconase and sodium cantharidate in vitro was assessed by combination indices and isobologram analyses. The experimental results show that the combination indices were less than 0.7, and the points of joint effect were located below the line of additivity. The combination of Onconase and sodium cantharidate exhibited a synergistic growth-inhibitory effect on both SPC-A-1 and A549 cell lines. The data of apoptotic cells studied by flow cytometry were consistent with above conclusion. The Onc/SCA combination may have potential in NSCLC therapy.