文献类型：期刊文献

英文题名：Characterization and Activity of Anthocyanins in Zijuan Tea (Camellia sinensis var. kitamura)

作者：Jiang, Lihua[1];Shen, Xiaojia[1];Shoji, Toshihiko[2];Kanda, Tomomasa[3];Zhou, Jiachun[1];Zhao, Liming[1]

机构：[1]E China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Natl Inst Fruit Tree Sci, Plant Physiol & Fruit Chem Div, Natl Agr & Food Res Org NARO, Tsukuba, Ibaraki 3058605, Japan;[3]Asahi Grp Holdings Ltd, Res Labs Fundamental Technol Food, Moriya, Ibaraki 3020106, Japan

年份：2013

卷号：61

期号：13

起止页码：3306

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20131516191251);WOS:【SCI-EXPANDED(收录号:WOS:000317259100018)】；

基金：This work is supported by the Fundamental Research Funds for the Central Universities, China.

语种：英文

外文关键词：Zijuan tea; anthocyanin; antioxidant activity; LC-ESI-MS; NMR

摘要：Zijuan tea is a new cultivar produced in Yunnan province of China. Unlike most tea cultivars, Zijuan tea is anthocyanin-rich. The composition and antioxidant activities of anthocyanins of Zijuan tea were studied for the first time in this paper. Anthocyanins were extracted with acidified methanol and quantified as 707 +/- 28 mu g/g of dry weight (cyanidin-3-O-beta-D-glucoside equivalent) by high-performance liquid chromatography (HPLC) analysis. Four anthocyanins were successfully identified after Amberlite XAD-7HP adsorption column chromatography and octadecyl silane (ODS) flash chromatography. Among the four, delphinidin-3-O-beta-D-galactoside (1) and cyanidin-3-O-beta-D-galactoside (2) were confirmed by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) and HPLC. Delphinidin-3-O-beta-D-(6-(E)-p-coumaroyl) galactopyranoside (3) and cyanidin-3-O-beta-D-(6-(E)-p-coumaroyl) galactopyranoside (4) were characterized by the high-resolution time-of-flight-mass spectrometry (HRTOF-MS) and nuclear magnetic resonance (NMR) spectrometry. The antioxidant activities of compounds 3 and 4, which composed approximately 75% of the total anthocyanins content in HPLC analysis, were evaluated by DPPH and FRAP assays. Results showed that both had higher antioxidant activities than commercial antioxidants butylated hydroxytoluene (BHT) used as one of controls in these assays.