文献类型：期刊文献

英文题名：Novel synthetic 2,6-dichloroisonicotinate derivatives as effective elicitors for inducing the biosynthesis of plant secondary metabolites

作者：Qian, Zhi-Gang[1]; Zhao, Zhen-Jiang[2]; Xu, Yufang[2]; Qian, Xuhong[2]; Zhong, Jian-Jiang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Inst Pesticides & Pharmaceut, Shanghai 200237, Peoples R China

年份：2006

卷号：71

期号：2

起止页码：164

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：2006259945713);WOS:【SCI-EXPANDED(收录号:WOS:000239207800005)】；

语种：英文

外文关键词：Bioassay - Biosynthesis - Derivatives - Metabolism - Metabolites - Plant cell culture

摘要：Two novel 2,6-dichloroisonicotinic acid (INA) derivatives [trifluoroethyl 2,6-dichloroisonicotinate (TFINA) and 2-(2,6-dichloro-pyridine-4-carbonyloxy)-ethyl jasmonate (DPCEJ)] were chemically synthesized and evaluated by bioassay as potential elicitors for inducing the biosynthesis of plant secondary metabolites. A suspension culture of Taxus chinensis, which stably produces a high level of bioactive taxuyunnanine C (Tc), was taken as a model plant cell system. A significant increase in Tc accumulation was observed in the presence of TFINA or DPCEJ. For example, addition of 100 mu M TFINA or DPCEJ on day 7 led to a high Tc content of 21.6 +/- 2.0 or 27.7 +/- 1.0 mg g(-1) (on day 21), while the Tc content was 13.7 +/- 1.0 and 17.1 +/- 0.9 mg g(-1) for the control and that with addition of 100 mu M INA, respectively. To the best of our knowledge, this is the first report on the use of synthetic INA derivatives for inducing the biosynthesis of plant secondary metabolites. The results indicate that the newly synthesized INA analogues can act as promising elicitors for secondary metabolism induction in plant cell cultures.