文献类型：期刊文献

英文题名：Multi-omic responses of fish exposed to complex chemical mixtures in the Shenandoah River watershed

作者：Bertolatus, David W.[1];Barber, Larry B.[2];Martyniuk, Christopher J.[3];Zhen, Huajun[4,8];Collette, Timothy W.[4];Ekman, Drew R.[4];Jastrow, Aaron[5];Rapp, Jennifer L.[6];Vajda, Alan M.[7]

机构：[1]Adams State Univ, Sch Sci Technol Engn & Math, 208 Edgemont Blvd, Alamosa, CO 81101 USA;[2]US Geol Survey, 3215 Marine St, Boulder, CO 80303 USA;[3]Univ Florida, Coll Vet Med, Ctr Environm & Human Toxicol, Dept Physiol Sci,Genet Inst, Gainesville, FL 32610 USA;[4]US EPA, Ctr Environm Measurement & Modeling, Athens, GA 30605 USA;[5]US EPA, Reg Lab Serv & Appl Sci Div 5, Chicago, IL 60605 USA;[6]US Geol Survey, Integrated Informat Disseminat Div, Decis Support Branch, 1730 East Parham Rd, Richmond, VA 23228 USA;[7]Univ Colorado, Dept Integrat Biol, CB 171, Denver, CO 80217 USA;[8]East China Univ Sci & Technol, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：902

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20233314546506);WOS:【SCI-EXPANDED(收录号:WOS:001063338100001)】；

基金：We would like to thank Wayne Pence (Virginia Department of Game and Inland Fisheries) , Dawn Liscomb and Tony Widmer (Virginia Department of Conservation and Resources) , Robert Hevener, Anita Riggleman, Sharon Foley (Harrisonburg/Rockingham Regional Sewer Authority) , Reid Wodicka, James Didawick (City of Woodstock, VA) , Quincy Teng and Jonathan Mosley (US EPA, Athens GA) , Bud Griswold, and Jordan Schmidt for their assistance making this research possible. We thank Don Tillet (U.S Geological Survey) for providing fathead minnows and helpful suggestions throughout the process. This work was funded by the U.S. Geological Survey Environmental Health Program. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The views expressed in this article are those of the authors and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency.

语种：英文

外文关键词：Endocrine disruption; Environmental mixtures; Landscape ecotoxicology; Ecotoxicogenomics; Transcriptomics; Metabolomics

摘要：To evaluate relationships between different anthropogenic impacts, contaminant occurrence, and fish health, we conducted in situ fish exposures across the Shenandoah River watershed at five sites with different land use. Exposure water was analyzed for over 500 chemical constituents, and organismal, metabolomic, and transcriptomic endpoints were measured in fathead minnows. Adverse reproductive outcomes were observed in fish exposed in the upper watershed at both wastewater treatment plant (WWTP) effluent-and agriculture-impacted sites, including decreased gonadosomatic index and altered secondary sex characteristics. This was accompanied with increased mortality at the site most impacted by agricultural activities. Molecular biomarkers of estrogen exposure were unchanged and consistent with low or non-detectable concentrations of common estrogens, indicating that alternative mechanisms were involved in organismal adverse outcomes. Hepatic metabolomic and transcriptomic profiles were altered in a site-specific manner, consistent with variation in land use and contaminant profiles. Integrated biomarker response data were useful for evaluating mechanistic linkages between contaminants and adverse outcomes, suggesting that reproductive endocrine disruption, altered lipid processes, and immunosuppression may have been involved in these organismal impacts. This study demonstrated linkages between human-impact, contaminant occurrence, and exposure effects in the Shenandoah River watershed and showed increased risk of adverse outcomes in fathead minnows exposed to complex mixtures at sites impacted by municipal wastewater discharges and agricultural practices.