文献类型：期刊文献

英文题名：Injectable sericin based nanocomposite hydrogel for multi-modal imaging-guided immunomodulatory bone regeneration

作者：Jiang, Li-Bo[1];Ding, Sheng-Long[1];Ding, Wang[1,4];Su, Di-Han[1];Zhang, Fang-Xue[1];Zhang, Tai-Wei[1];Yin, Xiao-Fan[4];Xiao, Lan[5];Li, Yu-Lin[3];Yuan, Feng-Lai[2];Dong, Jian[1]

机构：[1]Fudan Univ, Zhongshan Hosp, Dept Orthopaed Surg, 180 Fenglin Rd, Shanghai 200032, Peoples R China;[2]Jiangnan Univ, Hosp Affiliated, Inst Integrated Chinese & Western Med, Wuxi 214041, Jiangsu, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Engn Res Ctr Biomed Mat, State Key Lab Bioreactor Engn,Minist Educ, Shanghai 200237, Peoples R China;[4]Fudan Univ, Minhang Hosp, Dept Orthopaed Surg, 170 Xin Song Rd, Shanghai 201199, Peoples R China;[5]Queensland Univ Technol, Inst Hlth & Biomed Innovat, Australia China Ctr Tissue Engn & Regenerat Med A, 60 Musk Ave, Brisbane, Qld 4059, Australia

年份：2021

卷号：418

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20211310135112);WOS:【SCI-EXPANDED(收录号:WOS:000655688200004)】；

基金：This work was supported by the Shanghai "Rising Stars of Medical Talent" Youth Development Program(Youth Medical Talents -Specialist Program, [2020]087), Shanghai Talent Development Fund (2020067), National Natural Science Foundation of China (No. 81702177, 81770876, 81772317, 81972508, 81772855), and Natural Science Foundation of Jiangsu Province (Grant BK20191141).

语种：英文

外文关键词：Sericin; Injectable hydrogel; Osteoimmune microenvironment; Bone regeneration

摘要：Irregular bone defects, where the inflammation and immune microenvironment confronted with implanted biomaterials, remain a prominent challenge for bone regeneration. In this study, we fabricated an injectable alginate/sericin/graphene oxide (Alg/Ser/GO) hydrogel based on the Alg-Tyramine framework with HRP/H2O2 enzymatic crosslinking. This hydrogel exhibited bioimaging property and controlled degradation behavior upon releasing sericin and GO. Importantly, synergistic effects on bone regeneration between sericin and GO were demonstrated. GO significantly enhanced the spreading, osteogenic differentiation, and mineralization of encapsulated rat BMSCs, whereas the released sericin promoted M2 polarization and migration via the NF-kappa B and MAPK pathways. The M2 polarization of macrophages induced osteogenic differentiation of BMSCs via several secreted cytokines. Both in vivo and in vitro experiments showed that the Alg/Ser/GO hydrogel induced macrophage infiltration into the surrounding tissues and inhibited inflammation and fibrous capsule thickening. Last, the injected Alg/Ser/GO hydrogels with BMSCs promptly repaired established distal femoral defects in rats. Therefore, the fabricated Alg/Ser/GO hydrogel, along with macrophages and BMSCs, is a promising biomaterial for bone healing, especially the irregular bone defects.