文献类型：期刊文献

英文题名：Encapsulated feeder cells within alginate beads for ex vivo expansion of cord blood-derived CD34+ cells

作者：Pan, Xiuwei[1];Sun, Qiong[1];Cai, Haibo[1];Gao, Yun[1];Tan, Wensong[1];Zhang, Weian[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab Funct Mat Chem, Sch Mat Sci & Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：4

期号：10

起止页码：1441

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20163902852548);WOS:【SCI-EXPANDED(收录号:WOS:000384404000005)】；

基金：This work was financially supported by the National Natural Science Foundation of China (no. 51173044 and 21574039), the Research Innovation Program of SMEC (no. 14ZZ065), the Science and Technology Commission of Shanghai Municipality (no. 15JC1401402), the Shanghai Pujiang Program under 14PJ1402600 and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. W. Z. also acknowledges the support from the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Stem cells - Alginate - Feeding - Expansion

摘要：A co-culture system based on encapsulated feeder cells within alginate beads was developed through optimizing the detailed aspects of the cell culture system to expand CD34-positive (CD34(+)) cells ex vivo. Mesenchymal stem cells isolated from different sources (human amniotic (hAMSCs) and umbilical cord (UCMSCs)), and human fibroblast cells (HFs) have been respectively chosen as feeder cells and the results showed that the hAMSCs were superior to UCMSCs and HFs in conventional two-dimensional (2D) co-cultures regarding the promotion of total nucleated cell (TNCs) expansion and the maintenance of the CD34(+) phenotype. Alginate beads were employed to limit the growth of hAMSCs, which could effectively restrict the proliferation of the encapsulated hAMSCs, while the cell viability of hAMSCs was still highly maintained. Intriguingly, only a few hAMSCs migrated out of the alginate beads, whereas secreted growth factors and cytokines could be easily released. Furthermore, the alginate beads supported CD34(+) cells/hAMSCs 2D indirect co-culture exhibited increased TNCs expansion, higher percentages of CD34(+) and CD34(+) CD38-cells, and better cell vitality when compared to the 2D co-culture. Therefore, the co-culture system based on encapsulated hAMSCs within alginate beads can effectively promote CD34(+) cells to expand ex vivo.