文献类型：期刊文献

英文题名：Hematopoietic reconstitution of CD34+ cells derived from short-term cultured cord blood mononuclear cells

作者：Yang, Shi[1]; Cai, Haibo[1]; Jin, Huili[1]; Fan, Jinli[1]; Tan, Wen-Song[1]

机构：[1] The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200-237, China

年份：2009

卷号：14

期号：4

起止页码：429

外文期刊名：Biotechnology and Bioprocess Engineering

收录：EI(收录号：20094112371418)

语种：英文

外文关键词：Blood - Cytology - Stem cells - Mammals

摘要：Ex vivo expansion of hematopoietic stem cells (HSCs) is very important for clinical applications of cord blood (CB). With the aim to find proper culture duration for ex vivo expansion, mononuclear cells (MNC) was applied as starting culture cells to expand HSCs and the repopulating potential of seven-day and fourteen-day cultured CD34+ cells were compared. The average expansion of total cells and CD34+ cells cultured for 7 days were higher than those cultured for 14 days. The results of phenotypic analysis of fresh and cultured cells showed that the percentage of CD3+ cells declined and the percentage of CD33+ cells increased during culture. The engraftment levels of fourteen-day cultured CD34+ cells were higher than those of fresh and seven-day cultured CD34+ cells. Fourteen-day cultured CD34+ cells also showed better multilineage reconstitution ability than fresh and seven-day cultured CD34+ cells. The results of the present study demonstrated that prolonged culture could preserve the hematopoietic reconstitution ability of ex vivo cultured CB cells and improve the engraftment level in NOD/SCID mice. ? The Korean Society for Biotechnology and Bioengineering and Springer Berlin Heidelberg 2009.