文献类型：期刊文献

英文题名：A fluorogenic 2D glycosheet for the simultaneous identification of human- and avian-receptor specificity in influenza viruses

作者：Song, Jin-Xing[1,2];Tang, Xin-Ying[3];Zhou, Dong-Ming[3];Zhang, Wenqing[1,2];James, Tony D.[4];He, Xiao-Peng[1,2];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Inst Pasteur Shanghai, Vaccine Res Ctr, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China;[4]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2017

卷号：4

期号：3

起止页码：431

外文期刊名：MATERIALS HORIZONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000400837600015)】；

基金：This research was supported by the 973 project (2013CB733700), the National Natural Science Foundation of China (21572058 and 21576088), the Science and Technology Commission of Shanghai Municipality (15540723800), the Knowledge Innovation Program (Y014P31503), the Fundamental Research Funds for the Central Universities and the 100 Talent Program (Y316P11503) from the Chinese Academy of Sciences (to D. Z.), and the Shanghai Rising-Star Program (16QA1401400) (to X.-P. H.). Miss Ya-Li Zeng is warmly thanked for her contribution to the research. The Catalysis And Sensing for our Environment (CASE) network is thanked for research exchange opportunities. T. D. J. thanks ECUST for a guest professorship.

语种：英文

摘要：The switch in glycan-receptor specificity of influenza viruses may cause their interspecies transmission. However, tools that can simultaneous unveil the different receptor specificities of a single virus strain, in a homogeneous solution, have been elusive. Here we show a simple yet effective "2D glycosheet" that is capable of simultaneously identifying the human-and avian-glycan-receptor specificity of influenza viruses. Two fluorophores with different emission colors are coupled with avian-or human-glycan receptors. Then, the glycan-fluorophore conjugates with different receptors and emission colors are co-assembled on a 2D molybdenum disulfide platform, producing a duplexed, fluorogenic 2D glycosheet. While the system shows a single emission color with viruses containing a single receptor specificity, in the presence of a virus (H7N9) containing dual (human- and avian-) receptor specificity the system produces both emission colors. Interestingly, the use of graphene oxide and carbon nanotubes as the material substrate also produces dual emission with H7N9, thus enabling a new generation of low-dimensional glyco-materials for effectively probing the switch in receptor-specificity of influenza viruses.