文献类型：期刊文献

英文题名：A comparison on the preparation of hot water extracts from Chlorella pyrenoidosa (CPEs) and radical scavenging and macrophage activation effects of CPEs

作者：Zhuang, Xiuyuan[1];Zhang, Daojing[1];Qin, Wen[2];Deng, Jia[1];Shan, Hui[1];Tao, Liming[3];Li, Yuanguang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2014

卷号：5

期号：12

起止页码：3252

外文期刊名：FOOD & FUNCTION

收录：;EI(收录号：20144900272620);WOS:【SCI-EXPANDED(收录号:WOS:000345454700022)】；

基金：This work was supported by National Basic Research Program of China (973 Program: 2011CB200906) and National High Technology Research and Development Program of China (863 Program: 2014AA022004).

语种：英文

外文关键词：Water - Algae - Free radicals - Extraction - Nitric oxide - Absorbance - Chemical activation

摘要：Development of hot water extracts of Chlorella pyrenoidosa (CPE) may help in the reduction of the cost involved in C. pyrenoidosa-based biofuel production. The current extraction conditions of CPE greatly vary and the CPE-related research are developing rather slowly. To find an effective preparation method, in the present study, we have prepared CPEs using high pressures, ultrasonication, and enzymolysis, and we have compared the yields, general ingredients, and bioactivities of these products. The yields of the high pressure and enzymolysis methods were more than 25%. CPE-a, which has been prepared under an extra 0.1 MPa pressure in an autoclave, was rich in protein, and it had the strongest absorbance at 260 and 280 nm, whereas CPE-c and CPE-d, which were prepared via the enzymolysis of cellulase and pectinase, had higher sugar content. The CPEs had no inhibitory influence on the formation of advanced glycation end products, and their metal chelating activities were poor. However, all the products had significant positive effects on in vitro free radical scavenging and macrophage growth promotion. Hydroxyl radicals were scavenged in a concentration-dependent manner by CPE-a, and the cultured macrophage Ana-1 proliferated to 162.98% of the control when CPE-a was administrated at 200 mu g mL(-1). Furthermore, phagocytic activity and intracellular nitric oxide levels of Ana-1 were significantly enhanced with the administration of CPE-a. In brief, our results suggest that hot water extraction with high pressure is an effective method for preparing high value-added bio-products from C. pyrenoidosa, which have strong potential for use in free radical scavenging and macrophage activation.