文献类型：期刊文献

英文题名：Application of high-throughput screening in biocatalysis

作者：Ju, Xin[1]; Zhang, Jie[2]; Chan, Kui[1]; Liang, Xiaoliang[1]; Tao, Junhua[1]; Xu, Jian-He[2]

机构：[1] EnzymeWorks, Inc., Division of Research and Development, Zhangjiagang #603, Gangcheng Rd., Jiangsu, 215600, China; [2] East China University of Science and Technology, Biocatalysis and Synthetic Biotechnology, 130 Meilong Road, Shanghai, 200237, China

年份：2016

起止页码：35

外文期刊名：Applied Biocatalysis: From Fundamental Science to Industrial Applications

收录：EI(收录号：20182705419415)

语种：英文

外文关键词：Biochemistry - Synthetic biology - Hydrolases - Biochemical engineering

摘要：Biocatalysis is playing an increasing role for the production of chemicals. Due to ever-increasing importance of biosynthesis of chemicals, biocatalysts with better and superior functions are needed. As a result, efficient, robust, and high-throughput screening methods are essential. This chapter covers some of the methods used to screen ketoreductases, nitrilases, oxygenases, alcohol oxidases, epoxide hydrolases, lipases and esterases, transaminases, aldolases, and hydroxynitrile lyases (HNLs). In general, these methods can be divided into "screening", "selection", and "detecting" in the viewpoint of methodology. A high-throughput NMR system has been developed to facilitate high-hroughput genomic screening. Due to significant difference in fluorescent properties of 7-methoxy-4-(aminomethyl)-coumarin (MAMC) and 7-Hydroxy-4-(aminomethyl)-coumarin (HAMC), MAMC could work as a differential tool for high-throughput screening. Glycosyltransferases play important roles in biotransformation and synthetic biology. To improve the activity and selectivity of glycosyltransferases, protein engineering and high-throughput screening are promising methods. ? 2016 Wiley-VCH Verlag GmbH & Co. KGaA.