文献类型：期刊文献

英文题名：Determination of β-adrenergic agonists by hapten microarray

作者：Zuo, Peng[1];Zhang, Yin[1];Liu, Jie[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Dept Food Engn & Sci, Shanghai 200237, Peoples R China

年份：2010

卷号：82

期号：1

起止页码：61

外文期刊名：TALANTA

收录：;EI(收录号：20103213139164);WOS:【SCI-EXPANDED(收录号:WOS:000279488900009)】；

基金：This work was financially supported by China NSF (20627005), the Shanghai project (09JC1404100), the National Special Fund for SKLBE (2060204), Shanghai Shuguang Program (065G32), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：beta-Adrenergic agonist; Residues; Microarray; Multiplexed determination

摘要：The use of highly active beta-agonists as growth promoters is not appropriate because of the potential hazard for human and animal health. To investigate the residue level of these beta-agonists, hapten microarrays were employed for clenbuterol (CLB), ractopamine (RAC) and salbutamol (SAL) residue analysis. CLB, RAC and SAL conjugates were immobilized on the slides, which were precoated by agarose film to construct hapten microarrays, and then the corresponding monoclonal antibodies of these beta-agonists and the standards or samples were introduced for indirect competitive immunoassay. Finally, Cy3-labeled secondary antibody was employed to indicate the antigen-antibody complex. The fluorescence intensity of each spot was imaged and recorded, and the calibration curve of each analyte was obtained by plot fluorescence intensity against different standard concentrations. Compared to the ELISA, the hapten microarray method was more sensitive, which got the detection limits 0.09 mu g/L for CLB, 0.50 mu g/L for RAC, and 0.01 mu g/L for SAL What's more, with the recovery rate between 96.5% and 106.4%, and the coefficient of variation below 10%, the proposed hapten microarray method was shown to be both quantitative and reproducible. (C) 2010 Elsevier B.V. All rights reserved.