文献类型：期刊文献

中文题名：冻融法制备SOD脂质体

英文题名：PREPARATION OF SOD LIPOSOME BY FREEZE-THAW METHOD

作者：赵健[1];王富军[1];周玲[1];袁勤生[1]

机构：[1]华东理工大学

年份：1996

卷号：27

期号：8

起止页码：346

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：脂质体;SOD;冻融;制备

外文关键词：liposome, SOD, freeze-thaw method, Preparation

摘要：将经超声处理的磷脂与待包埋的ＳＯＤ溶液混匀，在液氮中深度冷冻后于３５℃融化，经超声处理、离心即得ＳＯＤ脂质体。ＳＯＤ活性包埋率约为３０％。本法操作方便，所需设备简单，可避免直接与有机溶剂接触，适用于生物活性物质的包埋和大规模生产。
The SOD liposomes were prepared by freeze-thaw method. The lipids were mixed with SOD solution and frozen at -196℃ in liquid nitrogen and left to thaw at 35℃. The thawed milky solution was sonicated and centrifuged, and the SOD liposomes were obtained. The encapsulation efficiency of this process is about 30%. The method is simple and is superior to others for avoiding organic solvent inactivation to biological substance.It can be used to encapsulate most biological molecules and be easily scaled-up.