文献类型：期刊文献

英文题名：β-Sheet-Assisted Fabrication of Drug-Loaded Gelatin/Silk Fibroin Nanoparticles for Spinal Cord Injury Treatment

作者：Guo, Jiangtao[1,2];Liu, Junjie[3];Wang, Mingwei[1,2];Zhang, Yuhua[1,2];Zhang, Ziyu[1,2];Cohen Stuart, Martien[1,2,4];Chen, Junchao[5];Tang, Weiping[5];Guo, Xuhong[1,2];Qi, Dahu[3];Liu, Tao[3]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Multiphase Mat Chem Engn, Shanghai 200237, Peoples R China;[3]Henan Prov Peoples Hosp, Dept Orthoped Surg, Zhengzhou 450003, Henan, Peoples R China;[4]Wageningen Univ & Res, Phys Chem & Soft Matter, NL-6708 WE Wageningen, Netherlands;[5]Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China

年份：2025

卷号：26

期号：11

起止页码：8064

外文期刊名：BIOMACROMOLECULES

收录：;EI(收录号：20261620509887);WOS:【SCI-EXPANDED(收录号:WOS:001596605600001)】；

基金：This work was financially supported by the Shanghai Pujiang Programme (23PJD024), the Henan Provincial Science and Technology Department Key Research and Development Projects (241111313400), the State Key Laboratory of Chemical Engineering (No. SKL-ChE-24C01), the National Natural Science Foundation of China (NSFC) for Young Scholars (22402119), the National Key Research and Development Program of China (2023YFD1700303), and the Science and Technology Innovation Program of Hunan Province (2024RC7001).

语种：英文

外文关键词：Biochemistry - Biocompatibility - Chemical stability - Controlled drug delivery - Crosslinking - Drug products - Fabrication - Nanoparticles - Proteins

摘要：Spinal cord injury (SCI) is a serious neurological disease accompanied by neuroinflammatory responses. Recently, nanosized drug delivery systems have attracted attention for SCI treatment by improving solubility and bioavailability and providing sustained drug release. Protein-based nanoparticles can be biocompatible but often require chemical cross-linkers for structural stability, raising biosafety concerns. Here, we introduce a beta-sheet-driven physical cross-linking approach to fabricate nanoparticles without chemical cross-linkers, namely by employing gelatin (Gel) and silk fibroin (SF) as proteins. This coassembly approach ensures structural integrity, enables enzyme-responsive degradation, and allows precise size control with narrow size distributions. Gel/SF particles can load dexamethasone (DEX) with a high drug loading (similar to 72%), exhibiting long-term stability and enzyme-triggered release. In vitro, these nanoparticles suppress pro-inflammatory cytokines and promote M2 phenotype microglial polarization, suggesting their neuroinflammation-regulating potential. Gel/SF particles may be a biocompatible, cross-linker-free protein-based drug delivery system, offering a promising platform for SCI treatment and broader applications in neuroinflammatory disorders.