文献类型：期刊文献

英文题名：Process Development for the Production of (R)-(-)-Mandelic Acid by Recombinant Escherichia coli Cells Harboring Nitrilase from Burkholderia cenocepacia J2315

作者：Wang, Hualei[1];Fan, Haiyang[1];Sun, Huihui[1];Zhao, Li[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：19

期号：12

起止页码：2012

外文期刊名：ORGANIC PROCESS RESEARCH & DEVELOPMENT

收录：;EI(收录号：20160101751634);WOS:【SCI-EXPANDED(收录号:WOS:000366878400025)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21406068/B060804), China Postdoctoral Science Foundation funded project (No. 2014M560308), and the National Basic Research Program of China (No. 2012CB721103).

语种：英文

外文关键词：Cost effectiveness - Organic acids - Hydrolysis

摘要：(R)-(-)-Mandelic acid is an important chiral building block that is widely used in pharmacy and the production of fine chemicals. A more advanced method for obtaining (R)-(-)-mandelic acid is direct hydrolysis of the corresponding racemic mandelonitrile. In order to develop a cost-effective process, a highly efficient enantioselective nitrilase BCJ2315 from Burkholderia cenocepacia J2315 was used for the biotransformation of mandelonitrile to (R)4)-mandelic acid. The recombinant Escherichia coli M15/BCJ2315 showed high substrate tolerance and could completely hydrolyze up to 250 mM of mandelonitrile. A fed-batch reaction was performed by periodically or continuously dosing the substrate into the reactor to alleviate substrate inhibition in a monophasic buffer system. Finally, the highest substrate loading (2.9 M) was achieved in the, continuous fed batch reaction mode, giving (R)-(-)-mandelic acid at the highest concentration (2.3 M, 350 g/L) with 97.4% ee ever reported. The hydrolysis process was easily Scaled up to 2 and 10 L, indicating the potential for the industrial production of optically pure (R)(-)-mandelic acid.