文献类型：期刊文献

英文题名：Study on the differences in sludge toxicity and microbial community structure caused by catechol, resorcinol and hydroquinone with metagenomic analysis

作者：Chen, Xiurong[1,2];Hu, Xueyang[1,2];Lu, Quanling[1,2];Yang, Yingying[1,2];Linghu, Shanshan[1,2];Zhang, Xinyu[1,2]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, Natl Engn Lab Ind Wastewater Treatment, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China

年份：2022

卷号：302

外文期刊名：JOURNAL OF ENVIRONMENTAL MANAGEMENT

收录：;EI(收录号：20233214514971);WOS:【SCI-EXPANDED(收录号:WOS:000719763200006)】；

基金：This research was supported by the National Key Research and Development Program of China (2017YFB0602804) , the Major Projects of Science and Technology (No. 2014ZX07202-011) in China, the Na-tional Natural Science Foundation (No. 51378207, No.51878278) , and the Shanghai Pujiang Programme (No. 13PJD009) , the Fundamental Research Funds for the Central Universities (222201817009) .

语种：英文

外文关键词：Dihydroxy benzene; Metagenomics; Sludge toxicity; Biodegradation pathway; Microbial community

摘要：The aerobic biodegradation rate, organic toxicity and microbial community structure of activated sludge acclimated by catechol, resorcinol and hydroquinone were investigated, to study the relationship between microbial structure and sludge organic toxicity caused by phenolic compounds. At the stable operation stage, the degradation rates of the dihydroxy benzenes in a single sequencing batch reactor (SBR) cycle were followed the order: resorcinol (89.71%) > hydroquinone (85.64%) > catechol (59.62%). Sludge toxicity bioassay indicated that the toxicity of sludge was catechol (45.63%) > hydroquinone (40.28%) > resorcinol (38.15%). The accumulation of secondary metabolites such as 5-10 kDa tryptophan and tyrosine protein substances caused the differential sludge toxicity. Microbial metagenomic analysis showed that the toxicity of sludge was significantly related to the microbial community structure. Thauera, Azoarcus, Pseudomonas and other Proteobacteria formed in the sludge during acclimation. Catechol group had the least dominant bacteria and loop ring opening enzyme genes (catA, dmpB, dxnF, hapD) numbers. Therefore, the degradation of catechol was the most difficult than resorcinol and hydroquinone, resulting the highest sludge toxicity.