文献类型：期刊文献

英文题名：Protective effects of hesperidin against oxidative stress of tert-butyl hydroperoxide in human hepatocytes

作者：Chen, Mingcang[1];Gu, Honggang[2];Ye, Yiyi[1];Lin, Bing[1];Sun, Lijuan[1];Deng, Weiping[1];Zhang, Jingzhe[2];Liu, Jianwen[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ Tradit Chinese Med, Longhua Hosp, Shanghai, Peoples R China

年份：2010

卷号：48

期号：10

起止页码：2980

外文期刊名：FOOD AND CHEMICAL TOXICOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000282543000062)】；

基金：This work was financially supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204) and the 111 Project (No.B07023) as well as Shanghai Committee of Science and Technology (No. 09JC1404500).

语种：英文

外文关键词：Hesperidin, Oxidative stress, tert-butyl hydroperoxide (t-BuOOH), heme; oxygenase-1 (HO-1); Extracellular signal-regulated protein kinase 1/2 (ERK 1/2); Nuclear factor erythroid 2 related factor (Nrf2)

摘要：Increasing evidence regarding free radical generating agents and the inflammatory process suggest that accumulation of reactive oxygen species (ROS) could involve hepatotoxicity. Hesperidin, a naturally occurring flavonoid presents in fruits and vegetables, has been reported to exert a wide range of pharmacological effects that include antioxidant, anti-inflammatory, antihypercholesterolemic, and anticarcinogenic actions. However, the cytoprotection and mechanism of hesperidin to neutralize oxidative stress in human hepatic L02 cells remain unclear. In this work, we assessed the capability of hesperidin to prevent tert-butyl hydroperoxide (t-BuOOH)-induced cell damage by augmenting cellular antioxidant defense. Hesperidin significantly protected hepatocytes against t-BuOOH-induced cell cytotoxicity, such as mitochondrial membrane potential (MMP) deplete and lactate dehydrogenase (LDH) release. Hesperidin also remarkably prevented indicators of oxidative stress, such as the ROS and lipid peroxidation level in a dose-dependent manner. Western blot showed that hesperidin facilitated ERK/MAPK phosphorylation which appeared to be responsible for nuclear translocation of Nrf2, thereby inducing cytoprotective heme oxygenase-1 (HO-1) expression. Based on the results described above, it suggested that hesperidin has potential as a therapeutic agent in the treatment of oxidative stress-related hepatocytes injury and liver dysfunctions. (C) 2010 Elsevier Ltd. All rights reserved.