文献类型：期刊文献

英文题名：Generation of rhBMP-2-induced juvenile ossicles in aged mice

作者：Dai, Kai[1,3];Shen, Tong[1,3];Yu, Yuanman[1,3];Deng, Shunshu[1,3];Mao, Lijie[1,3];Wang, Jing[1,3];Liu, Changsheng[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2020

卷号：258

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20203309043778);WOS:【SCI-EXPANDED(收录号:WOS:000568986400001)】；

基金：We thank R. Fu, X. Zhang, and C. Guo in the Shanghai Jiao Tong University School of Medicine (SHSMU) Flow cytometry Core for technical support; X. Ma in the East China Normal University (ECNU) Experimental Animal Center for technical support with rodent surgery and L. Jiang in the Fudan University State Key Laboratory of Molecular Engineering of Polymers for technical support with in vivo imaging assay. This research was supported by National Natural Science Foundation of China for Innovative Research Groups (No. 51621002) and the National Natural Science Foundation of China (No. 31870953).

语种：英文

外文关键词：Aging; Bone regeneration; rhBMP-2; Mesenchymal stromal cells; Type H vessels

摘要：Critical-sized bone defects and nonunions following fracture are common among elderly patients and severely reduce the quality of life. Dysfunctional senescent endothelial and mesenchymal stromal cells (MSCs) inhibit bone defect repair. Here we provide a method to obtain surrogate vascularized juvenile bone by subcutaneous implantation of recombinant human bone morphogenic protein-2 (rhBMP-2)-loaded absorbable gelatin scaffolds. RhBMP-2-induced ossicles showed fewer senenscent MSCs whereas much more type H blood vessels (strongly positive for CD31 and endomucin (Emcn)) and osteoprogenitor cells than native bone (femur and tibiae) even in old mice. Treatment with this juvenile ossicles improved the regenerative capacity in critical-sized cranial defects versus standard treatment in both young and old mice. Furthermore, ossicles with custom size shape were obtained by 3D-printing for irregular bone defects repair. These customizable juvenile ossicles developed in aged individuals provide an alternative to resecting native bone in autologous bone transplantation, with superior regenerative efficacy in elderly patients due to their juvenile phenotype.