文献类型：期刊文献

中文题名：Increasing NADPH impairs fungal H?O? resistance by perturbing transcriptional regulation of peroxiredoxin

作者：Jingyi Li[1];Yanwei Sun[1];Feiyun Liu[1];Yao Zhou[1];Yunfeng Yan[1];Zhemin Zhou[2];Ping Wang[3];Shengmin Zhou[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China;[2]Key Laboratory of Industrial Biotechnology(Ministry of Education),School of Biotechnology,Jiangnan University,1800 Lihu Avenue,Wuxi 214122,Jiangsu,China;[3]Department of Bioproducts and Biosystems Engineering,University of Minnesota,Twin Cities,Saint Paul,MN 55108,USA

年份：2022

卷号：9

期号：1

起止页码：1760

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：supported by the International S&T Innovation Cooperation Key Project(2017YFE0129600);the National Natural Science Foundation of China(21672065,22077032 and 21636003);the National Major Science and Technology Projects of China(2019ZX09739001);the Fundamental Research Funds for the Central Universities(22221818014);the 111 Project(B18022).

语种：英文

中文关键词：Peroxiredoxin;NADPH;Oxidative stress;Aspergillus;Glucose-6-phosphate dehydrogenase;AnCF

摘要：NADPH provides the reducing power for decomposition of reactive oxygen species(ROS),making it an indispensable part during ROS defense.It remains uncertain,however,if living cells respond to the ROS challenge with an elevated intracellular NADPH level or a more complex NADPH-mediated manner.Herein,we employed a model fungus Aspergillus nidulansto probe this issue.A conditional expression of glucose-6-phosphate dehydrogenase(G6PD)-strain was constructed to manipulate intracellular NADPH levels.As expected,turning down the cellular NADPH concentration drastically lowered the ROS response of the strain;it was interesting to note that increasing NADPH levels also impaired fungal H?O?resistance.Further analysis showed that excess NADPH promoted the assembly of the CCAAT-binding factor AnCF,which in turn suppressed NapA,a transcriptional activator of PrxA(the key NADPH-dependent ROS scavenger),leading to low antioxidant ability.In natural cell response to oxidative stress,we noticed that the intracellular NADPH level fluctuated“down then up”in the presence of H?O?.This might be the result of a co-action of the PrxA-dependent NADPH consumption and NADPH-dependent feedback of G6PD.The fluctuation of NADPH is well correlated to the formation of AnCF assembly and expression of NapA,thus modulating the ROS defense.Our research elucidated how A.nidulansprecisely controls NADPH levels for ROS defense.