文献类型：期刊文献

英文题名：Impact of Antifouling PEG Layer on the Performance of Functional Peptides in Regulating Cell Behaviors

作者：Chen, Qj[1];Yu, Shan[2];Zhang, Donghui[1];Zhang, Wenjing[1];Zhang, Haodong[1];Zou, Jingcheng[1];Mao, Zhengwei[2];Yuan, Yuan[1];Gao, Changyou[2];Liu, Runhui[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, State Key Lab Bioreactor Engn, Minist Educ,Key Lab Ultrafine Mat,Res Ctr Biomed, Shanghai 200237, Peoples R China;[2]Zhejiang Univ, Dept Polymer Sci & Engn, MOE Key Lab Macromol Synth & Functionalizat, Hangzhou 310027, Zhejiang, Peoples R China

年份：2019

卷号：141

期号：42

起止页码：16772

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20194107520309);WOS:【SCI-EXPANDED(收录号:WOS:000492800500031)】；

基金：We thank Dr. T. Wei, Dr. Q Yu, and Dr. H. Chen at Soochow University for assistance in ellipsometry characterization. We also appreciate the Research Center of Analysis and Test of East China University of Science and Technology for the help with the characterization. This research was supported by the National Key Research and Development Program of China (2016YFC1100401), the National Natural Science Foundation of China (No. 21774031), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the "Eastern Scholar Professorship" from Shanghai local government (TP2014034), the Natural Science Foundation of Shanghai (18ZR1410300), the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), and the Fundamental Research Funds for the Central Universities (22221818014).

语种：英文

外文关键词：Peptides - Chain length - Adhesives - Cells - Scaffolds (biology) - Cell engineering

摘要：Cell adhesive and other functional peptides (such as RGD, KRSR, YIGSR, VAPG, and BMP-2 peptides) are extensively studied and utilized in tissue engineering scaffolds and biomedical devices to modulate cell functions. Though PEG is frequently used as the antifouling layer, it is unclear how it affects the performance of functional peptides. By analyzing the impact of PEG at short (OEG4), medium (OEG8), and long chain length (PEG2K), we reveal that PEG chain length is critical and a medium-length PEG enables functional peptides to display their optimal and genuine functions in cell adhesion, migration, and differentiation by providing excellent antifouling to minimize background noise of unwanted cell adhesion and high enough surface density of functional peptides. Our result provides new avenues for maximizing the genuine functions of peptides. This study also provides a solution to prevent the heterogeneous and even divergent results caused by inappropriate choice of antifouling PEG and provides a general guidance in identifying new functional peptides.