文献类型：期刊文献

中文题名：A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells

英文题名：A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells

作者：Houna Duan[1];Yu Ding[1];Chusen Huang[2];Weiping Zhu[1];Rui Wang[3];Yufang Xu[1]

机构：[1]State Key Laboratory of Bioreactor Engineerring, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology;[2]The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry,College of Life and Environmental Sciences, Shanghai Normal University;[3]Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology

年份：2019

卷号：30

期号：1

起止页码：55

中文期刊名：Chinese Chemical Letters

外文期刊名：中国化学快报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2019_2020】；PubMed;

基金：the National Natural Science Foundation of China (No. 21476077) for financial support

语种：英文

中文关键词：Naphthalimide;Zinc;ions;Cellular;imaging;Lysosome;Fluorescent;probe

外文关键词：Naphthalimide;Zinc ions;Cellular imaging;Lysosome;Fluorescent probe

摘要：Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn2+binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn2+with the association constant of 4.9 ? 108 L/mol, and the detection limit of15 nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H2 O2 stimulation in SH-SY5 Y cells, endogenous release of Zn2+was observed.
Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn^(2+)binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn^(2+)with the association constant of 4.9×10~8 L/mol, and the detection limit of 15nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H_2O_2 stimulation in SH-SY5Y cells, endogenous release of Zn^(2+)was observed.