文献类型：期刊文献

英文题名：Fluorogenic probes for disease-relevant enzymes

作者：Zhang, Junji[1,2];Chai, Xianzhi[1,2];He, Xiao-Peng[1,2];Kim, Hae-Jo[3];Yoon, Juyoung[4];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Hankuk Univ Foreign Studies, Dept Chem, Yongin 17035, South Korea;[4]Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 03760, South Korea

年份：2019

卷号：48

期号：2

起止页码：683

外文期刊名：CHEMICAL SOCIETY REVIEWS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000465447700009)】；

基金：X. C., J. Z., X. P. H. and H. T. thank the National Natural Science Foundation of China (21788102, 21722801 and 21572058), the Programme of Introducing Talents of Discipline to Universities (B16017) and the Shanghai Rising-Star Program (16QA1401400). The Shanghai Municipal Science and Technology Major Project (2018SHZDZX03) and the international cooperation program of Shanghai Science and Technology Committee (17520750100) are warmly thanked for financially supporting international cooperations. H. J. K. acknowledges the financial support from the National Research Foundation of Korea (NRF 2017R1A2B4006706). J. Y. acknowledges a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (no. 2012R1A3A2048814).

语种：英文

摘要：Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in particular, the in situ activity of enzymes in live cells and in vivo. In this review, we comprehensively summarize recent progress that has been made in the development of small-molecule as well as material-based fluorogenic probes for sensitive detection of the activities of enzymes that are related to a number of human diseases. The principles utilized to design these probes as well as their applications are reviewed. Specific attention is given to fluorogenic probes that have been developed for analysis of the activities of enzymes including oxidases and reductases, those that act on biomacromolecules including DNAs, proteins/peptides/amino acids, carbohydrates and lipids, and those that are responsible for translational modifications. We envision that this review will serve as an ideal reference for practitioners as well as beginners in relevant research fields.