文献类型：期刊文献

中文题名：玉米秸秆水解液的黑曲霉发酵生产高浓度柠檬酸

英文题名：High Titer Citric Acid Fermentation from Corn Stover Hydrolysate by Aspergillus niger

作者：孟佼[1];周平平[1];张建[1];鲍杰[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2014

卷号：40

期号：6

起止页码：681

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家"973"计划(2011CB707406);国家"863"项目(2012AA022301;2014AA021901);国家自然科学基金(21306048;31300070);中央高校基本科研业务费专项资金(WF1214025;1314036)

语种：中文

中文关键词：柠檬酸;黑曲霉;玉米秸秆;木质纤维素

外文关键词：citric acid; Aspergillus niger ; corn stover; lignocellulose

摘要：柠檬酸是在饮料、制药、化妆品等领域有广泛应用的重要有机酸。利用玉米秸秆等木质纤维素原料生产柠檬酸可以显著降低其生产成本。目前,木质纤维素柠檬酸发酵的技术指标偏低,不具备工业应用价值。本文以黑曲霉Aspergillus niger SIIM M288为发酵菌株,以干法稀酸预处理和生物脱毒后获得的玉米秸秆酶水解液为原料,在优化条件下柠檬酸发酵质量浓度达到97.45g/L,相对于葡萄糖的得率达到了87.27%,是迄今为止所有报道的最高指标,已经接近以淀粉为原料的柠檬酸发酵指标。这一结果为以木质纤维素为原料生产柠檬酸的技术产业化提供了重要的依据。
Citric acid is one of the most versatile industrial organic acids in beverages, pharmaceutical, cosmetics, agriculture and biomedicine. Citric acid production using corn stover biomass as feedstock could provide an important way for cost reduction. The technical level of the reported citric acid producticn from lignocellulose biomass is far from the practical industry application. In this study, Aspergillus niger SIIM M288 was used to produce high titer citric acid using the corn stover hydrolysate as the substrate. Under the optimized condition, the citric acid titer and yield were achieved at 97. 45 g/L and 87. 27%, respectively, by Aspergillus niger SIIM M288 using corn stover hydrolysate. The results reached the highest level up to now. The results provided a possibility for economically citric acid production using the alternative lignocellulose feedstock.