文献类型：期刊文献

英文题名：Preparation of Polyvinyl Pyrrolidone Stationary Phase and its Application in Separation and Analysis of Flavonoids

作者：Chen, Xin[1]; Li, Juntao[2]; Gu, Mengting[1]; Miao, Yiyuan[1]; Ke, Yanxiong[1]; Zheng, Xiangwei[2]; Xu, Jian[3]

机构：[1] Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine, Ministry of Education, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; [3] Hubei Provincial Key Laboratory of Quality and Safety of Traditional Chinese Medicine Health Food, Jing Brand Research Institute, Jing Brand Co., Ltd., Hubei, 435100, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230076894)

语种：英文

外文关键词：Acetonitrile - Hydrogen bonds - Methanol - Silica

摘要：A reversed-phase/hydrophilic mixed-mode stationary phase (PVP@Silica) was prepared and applied to the separation of flavonoids. The difference in the Tanaka test results showed different retention mechanism of C18 column and PVP@Silica column. The retention behavior of flavonoids on PVP@Silica was investigated by a sample set containing 33 flavonoids with different substitution structures. It was observed that the retentions of conventional flavonoids on PVP@Silica column were much longer than those on C18 column due to the specific hydrogen bond interaction. In addition, (RP-PVP@Silica)-(RP-C18) system exhibited 60% orthogonality metric for these flavonoids with the methanol-water mobile phase. A U-shaped retention curve was observed in acetonitrile-water mobile phase system, indicating a change of retention mechanism from hydrophobic to hydrophilic with the increase of acetonitrile content. High orthogonality metric of 63.5% was achieved in (RP-PVP@Silica)-(HILIC-PVP@Silica) system. Finally, PVP@Silica was applied to the purification of total flavonoids in Ginkgo biloba extract. An off-line (RP-PVP@Silica)-(RP-C18) two-dimensional system with methanol-water mobile phase was demonstrated. ? 2023, The Authors. All rights reserved.