文献类型：期刊文献

英文题名：Magnetic Hydrogel: Enhanced Bacterial Biosensor for Speedy Gut Disease Detection

作者：Xu, Chu-Ying[1];Mi, Jing[2];Zhao, Jing-Feng[2];Zhu, Yan-Lin[2];Wu, Yu-Hou[2];Zhang, Chen-Rui[2];Zou, Zhen-Ping[2];Ye, Bang-Ce[1,2];Zhou, Ying[2]

机构：[1]Zhejiang Univ Technol, Coll Pharmaceut Sci, Yangtze River Delta Reg Green Pharmaceut, Collaborat Innovat Ctr,Inst Engn Biol & Hlth, Hangzhou 310014, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：10

期号：11

起止页码：8424

外文期刊名：ACS SENSORS

收录：;EI(收录号：20254919630688);WOS:【SCI-EXPANDED(收录号:WOS:001618575700001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (22134003), the National Key Research and Development Program of China (2023YFF1204500), and China Postdoctoral Science Foundation (2023M741176, 2024T170274).

语种：英文

外文关键词：bacterial biosensors; magnetic hydrogel; gutdisease; rapid diagnosis; intestinal inflammation

摘要：Bacterial biosensors represent a promising avenue for detecting gastrointestinal disease biomarkers; however, their direct oral delivery is limited by challenges such as bioavailability, safety, and interactions with the complex gut microbiota. To address these issues, we developed MagGel-BS, a Magnetic hydroGel-encapsulated Bacterial bioSensor platform. This hybrid system combines biocompatible alginate hydrogel to encapsulate both magnetic particles and bacterial biosensors, facilitating precise detection of disease biomarkers. The hydrogel significantly enhances stability, improving bacterial viability in gastric fluid by 10-fold. Utilizing heme-responsive bacteria, MagGel-BS was able to detect gastrointestinal bleeding in mice within only 20 min, a significant improvement compared to unencapsulated biosensors, which took several hours. Importantly, MagGel-BS showed no immune responses or adverse effects in vivo. In summary, this platform provides a rapid, noninvasive approach for gastrointestinal biomarker detection, demonstrating considerable potential for advancing bacterial-based diagnostics and therapeutics applications.