文献类型：期刊文献

英文题名：Metabolically Specific In Situ Fluorescent Visualization of Bacterial Infection on Wound Tissues

作者：Zhong, Chen-Jian[1,2,3];Hu, Xi-Le[4,5];Yang, Xiao-Lan[1,2,3];Gan, Hui-Qi[4,5];Yan, Kai-Cheng[4,5];Shu, Fu-Ting[2,3];Wei, Pei[1];Gong, Teng[1];Luo, Peng-Fei[2,3];James, Tony D.[6,7];Chen, Zhao-Hong[1];Zheng, Yong-Jun[2,3];He, Xiao-Peng[4,5,8,9];Xia, Zhao-Fan[1,3]

机构：[1]Fujian Med Univ Union Hosp, Fujian Burn Med Ctr, Dept Burn Surg & Wound Repair, Fujian Prov Key Lab Burn & Trauma, Fuzhou 350001, Fujian, Peoples R China;[2]Naval Med Univ, Dept Burn Surg, Affiliated Hosp 1, Shanghai 200433, Peoples R China;[3]Chinese Acad Med Sci, Res Unit Key Tech Treatment Burns & Combined Burns, Shanghai 200433, Peoples R China;[4]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China;[6]Univ Bath, Dept Chem, Bath BA2 7AY, England;[7]Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China;[8]Eastern Hepatobiliary Surg Hosp, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China;[9]Natl Ctr Liver Canc, Shanghai 200438, Peoples R China

年份：2022

卷号：14

期号：35

起止页码：39808

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20223712724082);WOS:【SCI-EXPANDED(收录号:WOS:000848493500001)】；

基金：This work was funded by the National Natural Science Foundation of China (91853201, 81930057, 21907030, and 82072170) , the Shanghai Science and Technology Committee (No. 19410712600) , CAMS Innovation Fund for Medical Sciences (2019-I2M-5-076) , Achievements Supportive Fund (2018-CGPZ-B03) , the Natural Science Foundation of Fujian Province (2021J01755) , the 14th Five-Year Plan for Medicine and Double High Construction Project of National Clinical Key Specialty of Fujian, China ( [2021]76) , and Fujian Provincial Key Laboratory of Burn and Trauma, China. T.D.J. thanks the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01) .

语种：英文

外文关键词：folate; bacteria; fluorescence imaging; wound infection; metabolism

摘要：The ability to effectively detect bacterial infection in human tissues is important for the timely treatment of the infection. However, traditional techniques fail to visualize bacterial species adhered to host cells in situ in a target-specific manner. Dihydropteroate synthase (DHPS) exclusively exists in bacterial species and metabolically converts p-aminobenzoic acid (PABA) to folic acid (FA). By targeting this bacterium-specific metabolism, we have developed a fluorescent imaging probe, PABA-DCM, based on the conjugation of PABA with a long-wavelength fluorophore, dicyanomethylene 4H-pyran (DCM). We confirmed that the probe can be used in the synthetic pathway of a broad spectrum of Gram-positive and negative bacteria, resulting in a significantly extended retention time in bacterial over mammalian cells. We validated that DHPS catalytically introduces a dihydropteridine group to the amino end of the PABA motif of PABA-DCM, and the resulting adduct leads to an increase in the FA levels of bacteria. We also constructed a hydrogel dressing containing PABA-DCM and graphene oxide (GO), termed PABA-DCM@GO, that achieves target-specific fluorescence visualization of bacterial infection on the wounded tissues of mice. Our research paves the way for the development of fluorescent imaging agents that target species conserved metabolic pathways of microorganisms for the in situ monitoring of infections in human tissues.