文献类型：期刊文献

英文题名：Biohydrogen production by a novel strain Petroclostridium sp. X23 isolated from the production water of oil reservoirs

作者：Fang, Bo[1,2,3];Liu, Yi-Fan[1,2,3];Pan, Xu-Jie[1,2];Zhou, Lei[1,2,3];Yang, Shi-Zhong[1,2,3];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Guangdong Technion Israel Inst Technol, Environm Engn, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China

年份：2024

卷号：228

外文期刊名：RENEWABLE ENERGY

收录：;EI(收录号：20242116113254);WOS:【SCI-EXPANDED(收录号:WOS:001243388100001)】；

基金：This work was supported by the National Natural Science Foundation of China (42061134011 and 42173076) , the Natural Science Founda- tion of Shanghai (21ZR1417400) , the Fundamental Research Funds for the Central Universities of China (JKJ01231714) , and the Research Program of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Biohydrogen production; Dark -fermentation; Petroclostridium; Pure culture; Sugar fermentation

摘要：Biohydrogen production from H2-producing has been considered a promising alternative for the replacement of fossil fuels. However, the mechanisms that affect the H2-producing capacities of wild-type strains remain largely elusive. To explore the capabilities of biohydrogen production from microorganisms in the deep subsurface oilreservoir environment, we isolated an H2-producing bacteria identified as the genus Petroclostridium from a longterm methanogenic crude oil-degrading enrichment culture of the production water from oil reservoir. The impact of various temperature, pH, and glucose concentrations on hydrogen production by Petroclostridium sp. X23 was investigated. Under the conditions of 37 degrees C, initial pH 8.0, and glucose concentration of 4 g/L, the strain X23 achieved its maximum hydrogen yield at 1.82 +/- 0.12 mol H2/mol glucose. When compared to the previously reported strain SK-Y3, X23 exhibited enhanced H2-producing ability, with lesser energy biomass production. Comparative genomic analysis revealed that the genome of X23 contains more genes associated with hydrogen generation than SK-Y3. These findings proposed Petroclostridium sp. X23 is a potential candidate for dark fermentative hydrogen production under alkaline and mesophilic conditions. It also increased our knowledge of genotypes related to high H2-producing capability, which may provide instructions for future selection of high-yielding strains and reasonable metabolic engineering of wild-type H2-producing strains.