文献类型：期刊文献

中文题名：新型聚乙烯醇系亲和吸附剂的制备及其在糖蛋白纯化中的应用(I)基质合成

英文题名：STUDY ON SYNTHESIS OF POLYVINYL ALCOHOL AFFINITY ADSORBENT AND ITS APPLICATION FOR PURIFYING GLYCOPROTEIN ( I ) MATRIX SYNTHESIS

作者：雷引林[1];刘坐镇[1];刘勤凤[1];宁方红[1];邬行彦[1]

机构：[1]生物反应器工程国家重点实验室华东理工大学华震公司,上海200237

年份：2000

卷号：16

期号：4

起止页码：344

中文期刊名：离子交换与吸附

外文期刊名：Ion Exchange and Adsorption

收录：北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金!(29706002)

语种：中文

中文关键词：亲和层析;纯化;糖蛋白;聚乙烯吸附剂

外文关键词：Vinyl acetate; Glycidyl methacrylate; Allyl methacrylate; Affinity Chromatography.

摘要：以醋酸乙烯酯(VAC)和甲基丙烯酸缩水甘油酯(GMA)为单体，甲基丙烯酸烯丙酯(AMA)为交联剂，在致孔剂正庚烷/乙酸丁酯的存在下，悬浮聚合，制得了一系列大孔共聚物；醇解后得到一种新型聚乙烯醇系亲和层析基质。依据单体的Q、e值，计算了体系的竞聚率，讨论了不同交联度、GMA含量、致孔剂用量及配比对基质孔结构和性能的影响。
A series of novel macroporous polyvinyl alcohol copolymers (VGA) were synthesized using Vinyl acetate (VAC) and Glycidyl methacrylate (GMA) as monomers and Allyl methacrylate (AMA) as crosslinking agent by suspension copolymerization under the existence of mixed porogenic agent of n-heptane and butyl acetate. The effects of the crosslinking degree, the amount of GMA and porogenic agent, and the proportion of porogenic agent on copolymer performance and pore structure were studied. The alcoholysis products of copolymers can be used to affinity chromatography as matrices because of its strong hydrophilicity. From the Q and e values determined by former investigaters, the reactive ratios of the ternary system of VAC-GMA-AMA were calculated according to Alfrey-Price's Q-e equation. The calculated results indicated the copolymerization of system was possible.