文献类型：期刊文献

英文题名：A novel efficient medium for chromogenic catalysis of tetramethylbenzidine with horseradish peroxidase

作者：Li, Meng[1];Huang, Xiang-Rong[1];Guo, Yi[1];Shang, Ya-Zhuo[1];Liu, Hong-Lai[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2017

卷号：28

期号：7

起止页码：1453

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000406726500017)】；

基金：We thank the National Natural Science Foundation of China (Nos. 21476072, 91334203) for supporting this work.

语种：英文

外文关键词：TMB; HRP; Chromogenic catalysis; Colorimetric assay; Ionic liquid; SDS

摘要：The traditional surfactant sodium dodecyl sulfate (SDS) and ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate ([Emim][BF4]) have been combined to create a novel efficient medium for chromogenic catalysis of 3,3',5,5'-tetramethylbenzidine with horseradish peroxidase in presence of H2O2. The results have shown the [Emim][BF4] in the mediums can promote the rate of formation of the blue chromogen, the SDS is responsible for the stabilization of the blue chromogen due to the electrostatic attraction between positively charged blue chromogen and the negatively charged surfactant. The SDS/[Emiml [EU4 combination not only enhance catalytic activity of HRP remarkably but also stabilize the blue chromogen formed in the HRP oxidation of the substrate TMB compared to the conventional medium. Based on the superior combination of SDS and [Emim][BF4], the colorimetric assay for detecting HRP activity and H2O2 concentration was established. This work demonstrates a novel efficient medium for chromogenic catalysis with potential applications in biosensors and clinical diagnosis. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.