文献类型：期刊文献

中文题名：冬虫夏草(Cordyceps sinensis)菌丝体固体发酵粉中麦角甾醇的定量分析

英文题名：Analysis of ergsterol of Cordyceps Sinensis mycelial fermentation preparations in solid media using RP-HPLC

作者：王尊生[1];王升厚[1];袁勤生[2]

机构：[1]沈阳师范大学化学与生命科学学院,辽宁沈阳110034;[2]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2005

卷号：23

期号：3

起止页码：293

中文期刊名：沈阳师范大学学报（自然科学版）

外文期刊名：Journal of Shenyang Normal University:Natural Science Edition

收录：CSTPCD

语种：中文

中文关键词：RP-HPLC;麦角甾醇;固体发酵粉

外文关键词：RP-HPLC;ergosterol;fermentation preparations

摘要：建立了冬虫夏草固体发酵粉中以麦角甾醇含量为生物量指标的分析方法:麦角甾醇含量的RP-HPLC检测方法.采用超声和回流等方法处理样品.结果:虫草粉的乙醇提取物中游离麦角甾醇的含量为53333±603μg/g,总麦角甾醇的含量138146±1896μg/g,HPLC分析采用麦角甾醇标准品外标定量法,线性范围:057～14317μg/mL,直线回归分析方程:Y=1879X-2E-13,R2=1.精密度相对偏差(RSD):0361%.样品中麦角甾醇的含量稳定,重现性好,可以作为冬虫夏草固体发酵的生物量的控制指标.
With reversed-phase high-performance liquid chromatography (RP-HPLC),a method to determine ergosterol in extract of Cordyceps Sinensis mycelium solid fermentation preparations had been developed.The fermentation preparations were extracted by ultrasonic or circumfluence.The dissociative ergosterol was 533.33±6.03?μg,and total ergosterol was 1?381.46±18.96?μg.The linear range of the HPLC method was 0.57～143.17?μg/mL.The regressed equation was Y=18.79X-2E^(-13),R^2=1.The precision RSD was 0.361%.The ergosterol was stable and replicated well in the samples.it could be used as a control parameters in the determining of biomass of the solid fermentation preparations.