文献类型：期刊文献

英文题名：High frequency transformation of the industrial erythromycin-producing bacterium Saccharopolyspora erythraea

作者：Wang, Yong[1,2]; Wang, YiGuang[1,2]; Zhang, Siliang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, No. 1 Tiantan Xili, Beijing 100050, China

年份：2008

卷号：30

期号：2

起止页码：357

外文期刊名：Biotechnology Letters

收录：EI(收录号：20080211011040)

语种：英文

外文关键词：Bacteria - DNA sequences - Escherichia coli

摘要：The DNA transformation in the industrial erythromycin-producing Saccharopolyspora erythraea was investigated as standard protoplast transformation methods are ineffective. Intergeneric conjugal transfer of DNA from E. coli demonstrated transformation efficiencies from 0.05 × 10 -8 to 7.2 × 10-8 exconjugants generated per recipient. Electroporation-mediated methodologies were also established. More than 105 transformants were acquired per μg DNA. The proposed protocol provides an alternative route for the introduction of DNA into industrial strains. ? 2007 Springer Science+Business Media B.V.