文献类型：期刊文献

英文题名：A viscoelastic PEGylated poly(glycerol sebacate)-based bilayer scaffold for cartilage regeneration in full-thickness osteochondral defect

作者：Lin, Dan[1,2,3];Cai, Bolei[1,2,4];Wang, Le[4];Cai, Lisha[3];Wang, Zihao[3];Xie, Jirong[5];Lv, Qian-xin[4];Yuan, Yuan[3];Liu, Changsheng[3];Shen, Steve G. F.[1,2,6]

机构：[1]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral & Craniomaxillofacial Surg,Shanghai Key, Coll Stomatol,Sch Med,Natl Clin Res Ctr Oral Dis, Shanghai 200011, Peoples R China;[2]Shanghai Res Inst Stomatol, Shanghai 200011, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, State Key Lab Bioreactor Engn, Key Lab Ultrafine Mat,Engn Res Ctr Biomat, Shanghai 200237, Peoples R China;[4]Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, State Key Lab Mil Stomatol, Xian 710032, Peoples R China;[5]Jiamusi Univ, Sch Stomatol, Dept Prosthodont, Jiamusi 154003, Peoples R China;[6]Shanghai Univ Med & Hlth Sci, Shanghai 201318, Peoples R China

年份：2020

卷号：253

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20231313812795);WOS:【SCI-EXPANDED(收录号:WOS:000536893800003)】；

基金：The authors gratefully acknowledge the support of the National Natural Science Foundation of China (No. 81970973), National Key R&D Program of China (No. 2017YFB1104100), the National Natural Science Foundation of China for Innovative Research Groups (No.51621002), National Natural Science Foundation of China (No. 81570947, No. 81771036, No. 31470924, No. 31330028, and No. 81502338), Shanghai Sailing Program (19YF1425500), and Leading talents in Shanghai in 2017.

语种：英文

外文关键词：Viscoelastic PEGylated poly(glycerol sebacate); Cartilage regeneration; Osteochondral regeneration; Full-thickness articular defects; Bilayer scaffold

摘要：Defects of either articular cartilage or subchondral bone would destroy the structural integrity and functionality of the joint. Reconstruction of osteochondral defects requires difunctional scaffolds that simultaneously induce cartilage and subchondral bone morphogenesis, however, high-performance cartilage reconstructive scaffolds remain a considerable challenge. In this study, a solvent-free urethane crosslinking and spontaneous poreforming procedure under room temperature was proposed and optimized to produce PEGylated poly(glycerol sebacate) (PEGS) scaffolds with controllable crosslinking degrees and hierarchical macro-/micro-porosities. Based on the economical and feasible preparative approach, the viscoelastic PEGS-12h with low crosslinking degree was demonstrated to significantly stimulate chondrogenic differentiation, maintain chondrocyte phenotype and enhance cartilage matrix secretion compared to elastic polymer with high crosslinking degree, emphasizing the importance of matrix viscoelasticity in cartilage regeneration. On this basis, the viscoelastic low-crosslinked PEGS-12h was combined with the well-acknowledged osteoinductive mesoporous bioactive glass (MBG) to construct a difunctional PEGS/MBG bilayer scaffold, and evaluated in a full-thickness osteochondral defect model in vivo. The PEGS/MBG bilayer scaffold successfully reconstructed well-integrated articular hyaline cartilage and its subchondral bone in 12 weeks, exhibiting extraordinary regenerative efficiency. The results indicated that the viscoelastic PEGS scaffold and PEGS/MBG bilayer scaffold proposed in this study made an excellent candidate for cartilage and osteochondral regeneration, and was expected for clinical translation in the future.