文献类型：期刊文献

英文题名：Development, validation and application of a hydrophilic interaction liquid chromatography-evaporative light scattering detection based method for process control of hydrolysis of xylans obtained from different agricultural wastes

作者：Li, Fangbing[1];Wang, Hui[1];Xin, Huaxia[1];Cai, Jianfeng[1];Fu, Qing[1];Jin, Yu[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China

年份：2016

卷号：212

起止页码：155

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20162502512114);WOS:【SCI-EXPANDED(收录号:WOS:000378757800021)】；

基金：This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130074120017), and the Natural Science Foundation of Shanghai, China (Grant No. 13ZR1453200).

语种：英文

外文关键词：Xylooligosaccharides; Hydrophilic interaction liquid chromatography-evaporative light scattering detection; Process control; Agricultural wastes; Hydrolysis

摘要：Purified standards of xylooligosaccharides (XOSs) (DP2-6) were first prepared from a mixture of XOSs using solid phase extraction (SPE), followed by semi-preparative liquid chromatography both under hydrophilic interaction liquid chromatography (HILIC) modes. Then, an accurate quantitative analysis method based on hydrophilic interaction liquid chromatography-evaporative light scattering detection (HILIC-ELSD) was developed and validated for simultaneous determination of xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6). This developed HILIC-ELSD method was applied to the comparison of different hydrolysis methods for xylans and assessment of XOSs contents from different agricultural wastes. The result indicated that enzymatic hydrolysis was preferable with fewer by-products and high XOSs yield. The XOSs yield (48.40%) from sugarcane bagasse xylan was the highest, showing conversions of 11.21 g X2, 12.75 g X3, 4.54 g X4, 13.31 g X5, and 6.78 g X6 from 100 g xylan. (C) 2016 Elsevier Ltd. All rights reserved.