文献类型：期刊文献

英文题名：Glass carbon electrode modified with horseradish peroxidase immobilized on partially reduced graphene oxide for detecting phenolic compounds

作者：Zhang, Yan[1];Zhang, Jiali[1];Wu, Haixia[1];Guo, Shouwu[1];Zhang, Jingyan[2]

机构：[1]Shanghai Jiao Tong Univ, Natl Key Lab Micro Nano Fabricat Technol, Res Inst Micro Nano Sci & Technol, Key Lab Thin Film & Microfabricat,Minist Educ, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：681

起止页码：49

外文期刊名：JOURNAL OF ELECTROANALYTICAL CHEMISTRY

收录：;EI(收录号：20123215310673);WOS:【SCI-EXPANDED(收录号:WOS:000308054400008)】；

基金：This work was financially supported by the NSFC (No. 91123011, 90923041, 31070742), Shanghai Committee of Science and Technology (Grant 11DZ2260600), and the National "973" Program (2010CB933900) of China.

语种：英文

外文关键词：Partially reduced graphene oxide; Horseradish peroxidase; Glass carbon electrode; Phenolic compounds

摘要：A glass carbon (GC) electrode has been modified with horseradish peroxidase (HRP) molecules, which are immobilized on the partially reduced graphene oxide (PCRG). The surface properties of the as-modified electrode are characterized with scanning electron microscopy (SEM), the electrochemical characteristics of the as-modified electrode are studied using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). We demonstrated that the PCRG can promote the electron transfer between HRP and GC electrode, and the immobilized HRP maintained its catalytic activity of the decomposition of phenol and p-chlorophenol. The GC electrode modified with PCRG immobilized HRP exhibits better electrochemical property over CRC, the modified electrode may find practical application as enzyme-based amperometric sensors used for detections of phenolic molecules or other permanent organic pollutants in water. The method provides a strategy for preparation of a sensitive amperometric sensor for the detection of phenolic compounds and other permanent organic pollutants. (C) 2012 Elsevier B.V. All rights reserved.