文献类型：期刊文献

英文题名：Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH4 + concentration

作者：Zhang, Jianguo[1]; Wang, Xuedong[1]; Zheng, Yu[1]; Fang, Guochen[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2008

卷号：31

期号：2

起止页码：63

外文期刊名：Bioprocess and Biosystems Engineering

收录：EI(收录号：20080511067041)

语种：英文

外文关键词：Cell growth - Concentration (process) - Plants (botany)

摘要：Yield of S-adenosylmethionine was improved significantly in recombinant Pichia pastoris by controlling NH4+ concentration. The highest production rate was 0.248 g/L h when NH4+ concentration was 450 mmol/L and no repression of cell growth was observed. Within very short induction time (47 h), 11.63 g/L SAM was obtained in a 3.7 L bioreactor. ? 2007 Springer-Verlag.