文献类型：期刊文献

英文题名：Target-site directed rational high-throughput screening system for high sophorolipids production by Candida bombicola

作者：Chen, Yang[1];Tian, Xiwei[1];Li, Qianhui[1];Li, Ya[1];Chu, Ju[1];Hang, Haifeng[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：315

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20203008966109);WOS:【SCI-EXPANDED(收录号:WOS:000560716900003)】；

基金：This work was financially supported by the National Key Research and Development Program, China (2018YFA0900300, 2019YFA0904803, 2017YFB0309302), and the Fundamental Research Funds for the Central Universities, China (WF1814032, 22221817014, 22221818014). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Candida bombicola; Sophorolipids; Combined mutagenesis; Relaxation culture; Combined pressure

摘要：In this study we have established a rational and high performance high-throughput screening system to select for a sophorolipids (SLs) high-producing strain of Candida bombicola. Introduction of mutagen combination, relaxation culture and multi-stress significantly improved both mutation and positive mutation rates. A highperforming strain, Ncbio 5, was selected out of 6212 mutants. Final SLs titer, productivity and yield of this strain in 5 L bioreactors were 26.9%, 27.0% and 35.0% higher than in the original strain, respectively. The improved fermentation performance in Ncbio 5 is contributed by the longer production period, higher SLs productivity and more efficient oil utilization. The strategy adopted herein to optimize the high-throughput screening system should be readily extendable to other similar systems.