文献类型：期刊文献

英文题名：Dip-and-Read Method for Label-Free Renewable Sensing Enhanced Using Complex DNA Structures

作者：Zhang, Min[1];Jiang, Xiao-Qin[1];Le, Huynh-Nhu[1];Wang, Ping[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Biomed Nanotechnol Ctr, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2013

卷号：5

期号：3

起止页码：473

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20130816035890);WOS:【SCI-EXPANDED(收录号:WOS:000315079700001)】；

基金：This study is supported by the China NSF 21075040, the Shanghai Fund (11nm0502500, 11XD1401900), SRFDP (No. 20120074110009) and the Key Grant Project (No. 313019) of Chinese Ministry of Education, and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：DNA tetrahedron; aptamer; DNA detection; signal amplification; dip-and-read; label-free

摘要：A label-free assay is reported in this work for the detection of DNA with enhanced sensitivity using complex DNA structures (DNA tetrahedrons) based on the biolayer interferometry. The DNA tetrahedrons help to amplify the optical signals of the biolayer interferometry, thus improving the detection limit of DNA by about 100-fold. We further demonstrated that this method could be expanded to ATP detection by taking advantage of the target-dependent adaptability of aptamers. It appears to us that this new label-free assay promises new opportunities for developing novel biolayer interferometry assays.