文献类型：期刊文献

中文题名：用膜技术解除螺旋霉素生物合成过程中产物的反馈抑制作用

英文题名：ELIMINATION OF DETRIMENTAL METABOLITES INHIBITION DURING BIOSYNTHESIS OF SPIRAMYCIN BY MEMBARAN DIALYSIS

作者：李友荣[1];邹崇达[2]

机构：[1]华东化工学院生化工程系,上海200237;[2]华侨大学,泉州462011

年份：1992

卷号：17

期号：1

起止页码：6

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：螺旋霉素;发酵;膜透析

外文关键词：Spiramycin; Fermentation; Membrane dialysis

摘要：膜技术用于解除初级代谢物发酵过程中的终产物和其他有害代谢物的抑制作用已有许多报道,但应用于次级代谢物上则少见。本研究采用发酵液于罐外经透析装置循环的办法将其部分产物和有害代谢物转移到透析液中。以期提高菌的生产力和螺旋霉素的总产率。试验结果表明,采用膜透析方法与不用透析为对照相比,可以使菌在后期的比生产速率维持较高水平,从而使总产率提高30～50％,且透析发酵的生长与代谢曲线比对照波动性小,其最适透析条件为:透析膜孔径为3.0μm;发酵液与透析液体积比为0.9～1.0;开始透析时间为60h。
Membrane technology has been used chiefly to relief end product and toxicmetabolites inhibition during fermentation. However, its application to secondary metabolites fermentation was hardly reported. In this study, fermentation broth was circulated through a dialysis device to eliminate detrimental metabolites from the broth, so as to enhance the productivity of spiramycin.Experimental results show that the specific production rate was maintain at higher level and the total yield of spiramycin was increased by 30~50% with dialysis fermentation; besides, the fluctuation of growth and metabolic curves reduced evidently after applyng dialysis. Best yields were obtained under following conditions: membrane pore size,3.0μm; time for initiating dialysis, 60 h(after inoculation); ratio of working volume of culture uroth to dialysate, 1:1.