文献类型：期刊文献

英文题名：Biospecific Self-Assembly of a Nanoparticle Coating for Targeted and Stimuli-Responsive Drug Delivery

作者：Li, Jinyang[1,2,5];Qu, Xue[1,2];Payne, Gregory F.[3,4];Zhang, Cheng[2];Zhang, Yuxin[1,2];Li, Jianbo;Ren, Jie[5];Hong, Hua[1,2];Liu, Changsheng[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]Inst Biosyst & Biotechnol Res, College Pk, MD 20742 USA;[4]Fischell Dept Bioengn, College Pk, MD 20742 USA;[5]Tongji Univ, Sch Mat Sci & Engn, Inst Nano & Biopolymer Mat, Shanghai 201804, Peoples R China

年份：2015

卷号：25

期号：9

起止页码：1404

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20151000594908);WOS:【SCI-EXPANDED(收录号:WOS:000350541700010)】；

基金：The authors acknowledge the financial support from the National Basic Research Program of China (2012CB933600), the National Natural Science Foundation of China (51103043), the 111 project (B14018), Shanghai Natural Science Foundation (11ZR1409100), Innovation Program of Shanghai Municipal Education Commission (14ZZ060), the Fundamental Research Funds for the Central Universities (WD1214056), Shanghai Science and Technology Development Funds (14QA1401000), and the Robert W. Deutsch Foundation.

语种：英文

外文关键词：Cells - Glycoproteins - Cancer cells - Cell culture - Diseases - Proteins - Medical nanotechnology - Self assembly - Silica nanoparticles

摘要：Biology provides a range of materials, mechanisms, and insights to meet the diverse requirements of nanomedicine. Here, a biologically based nanoparticle coating system that offers three characteristic features is reported. First, the coating can be self-assembled through a noncovalent biospecific interaction mechanism between a lectin protein (Concanavalin A) and the polysaccharide glycogen. This biospecific self-assembly enables the coating to be applied simply without the generation of covalent bonds. Second, glycoprotein-based biofunctionality can be incorporated into the coating through the same noncovalent biospecific interaction mechanism. Here, the glycoprotein transferrin is incorporated into the coating since this moiety is commonly used to target cancer cells through a receptor-mediated endocytosis mechanism. Third, the coating can be triggered to disassemble in response to a reduction in pH that is characteristic of endosomal uptake. In a proof-of-concept study, comparing coated and uncoated nanoparticles, model drug-loaded nanoparticles (doxorubicin-loaded mesoporous silica nanoparticles) are prepared and it is observed that the coated nanoparticle has enhanced cytotoxicity for cancer cell lines but attenuated cytotoxicity for noncancerous cell lines. These studies demonstrate that biology provides unique materials and mechanism appropriate to meet the needs for emerging applications in the medical and life sciences.