文献类型：期刊文献

英文题名：Antifungal activity of Streptomyces albidoflavus L131 against the leaf mold pathogen Passalora fulva involves membrane leakage and oxidative damage

作者：Chen, Chao[1];Wang, Yumei[1];Su, Chun[1];Zhao, Xinqing[1];Li, Ming[2];Meng, Xiaowei[1];Jin, Yingyu[3];Yang, Seung-Hwan[3];Ma, Yushu[4];Wei, Dongzhi[4];Suh, Joo-Won[3]

机构：[1]Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China;[2]Dalian Med Univ, Dept Microecol, Dalian 116044, Peoples R China;[3]Myongji Univ, Dept Biol Sci, Inst Biosci & Biotechnol, Yongin 449728, South Korea;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：58

期号：1

起止页码：111

外文期刊名：JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000354354700016)】；

基金：This work was supported by Open Funding Project of State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, China. This work was also partially supported by the Fundamental Research program from the education department of Liaoning Province (L2012319), China.

语种：英文

外文关键词：Marine-derived Streptomyces albidoflavus; Tomato leaf pathogen; Passalorafulva (syn. Fulvia fulva Cladosporium fulvum); Antifungal activity; Reactive oxygen species (ROS)

摘要：Passalora fulva (or Fulvia fulva) is the causal microorganism of tomato leaf mold, the outbreak of which occurs worldwide in greenhouse especially when humidity is high. However, studies on antifungal agents of P. fulva are still very limited. In this study, a marine-derived Streptomyces albidoflavus strain L131 showing potent inhibitory activities against P. fulva was identified and characterized. The active antifungal components were obtained, and studies on the antifungal mechanisms of the crude extract showed that the antifungal metabolites of L131 caused damage of hyphae and spore development, as well as plasma membrane of P. fulva. In addition, accumulation of endogenous reactive oxygen species of the leaf pathogen was also observed after treatment by culture extracts of L131. To our knowledge, this is the first report on the studies of the antifungal mechanisms against P. fulva, which benefit further development of biocontrol agent against tomato leaf mold disease.