文献类型：期刊文献

英文题名：Dopant-driven fibrinogen self-assembly on titanium surfaces: Contrasting effects of calcium doping and calcium/silver co-doping

作者：Ahmed, Zubair[1];Yang, Nan[2];Qin, Hui[2];Cao, Huiliang[1,3,4]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Interfacial Electrochem & Biomat, Shanghai 200237, Peoples R China;[2]Shanghai Jiaotong Univ Affiliated Peoples Hosp 6, Dept Orthoped, Shanghai 200233, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2026

卷号：266

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20262020737593);WOS:【SCI-EXPANDED(收录号:WOS:001778250300001)】；

基金：This work was jointly supported by grants from the National Natural Science Foundation of China (32271399 and 31870945) , the Natural Science Foundation of Shanghai (21ZR1415700) , and the Shanghai Committee of Science and Technology (23S31901700) .

语种：英文

外文关键词：Protein adsorption; Fibrin; Calcium oxide; Silver nanoparticles; Titanium

摘要：Fibrinogen adsorption conditions the biological responds to implantable medical devices; however, this effect is surface chemistry dependent and associated mechanism is unclear. Here, it is shown that fibrinogen selfassembly distinctively on doped titanium because of its different binding sites to calcium ions and immobilized silver nanoparticles. Calcium doping and silver-calcium co-doping are applied on pure titanium by plasma technology, and their effects on fibrinogen adsorption are detailed by using X-ray photoelectron spectroscopy (XPS), dye-assisted scanning electron microscopy (d-SEM), and scanning electrochemical microscopy (SECM). It demonstrates that calcium-doping induces mesh-like fibrinogen assembly via calcium ion mediated conformational activation, particularly chelating with the histidine and carboxylate residues of the B(3 chain segment GlyHis-Arg-Pro ((315-(318). Conversely, silver addition disrupts calcium chelation by preferentially coordinating with cysteine thiol groups in Ac chain (Ac 161-165), and produces a dense fibrinogen layer on titanium. Since fibrinogen adsorption is a native action during medical device placement, the present study provides new insights into advancing surface modification of implantable biomaterials to orchestrate tissue integration at the protein and cellular levels.