文献类型：期刊文献

英文题名：Chrominance to Dimension: A Real-Time Method for Measuring the Size of Single Gold Nanoparticles

作者：Jing, Chao[1,2];Gu, Zhen[1,2];Ying, Yi-Lun[1,2];Li, Da-Wei[1,2];Zhang, Lei[1,2,3];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]Nanjing Univ Posts & Telecommun, Sch Mat Sci & Engn, Nanjing 210046, Jiangsu, Peoples R China

年份：2012

卷号：84

期号：10

起止页码：4284

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20122115044145);WOS:【SCI-EXPANDED(收录号:WOS:000303965500009)】；

基金：This research was supported by the National Science Fund for Distinguished Young Scholars (21125522), the National Natural Science Foundation of China (91027035, 21007015), the Fundamental Research Funds for the Central Universities (WK1013002) and the Open Project Program of the State Key Laboratory of Chemical Engineering (ECUST, SKL-ChE-11C01). We especially thank Li Ni, Jin Huang and Tao Xie from ECUST for the Matlab programming.

语种：英文

外文关键词：Particle size - Color - Fiber optic sensors - Light scattering - MATLAB - Light - Scanning electron microscopy - Metal nanoparticles - Refractive index

摘要：Noble metal nanoparticles have excellent optical and chemical properties and are widely used in optics, sensors, and biomedicines. The inherent characteristics of metal nanoparticles, particularly their size, play important roles in their applications. The ability to readily measure the size of single nanomaterials on-site is crucial to the rapid development of single-particle sensors. In this study, we developed a facile and real-time method for estimating the diameter of single gold nanoparticles (GNPs) that range from 35 to 110 nm in diameter; this technique uses the chrominance of the GNP's plasmon resonance scattering light that is captured by a dark-field microscope (DFM). The RGB (three primary colors, red, green, and blue) chrominance information from the dark-field image can be directly converted into the diameters of the GNPs using the relationship between the particle size and the scattering light peak wavelength; this conversion was carried out using Mat lab program based on an RGB-To-Wavelength (RTW) process. This approach is more convenient, less time-consuming, and enables observation under arbitrary conditions compared to the scanning electron microscopy (SEM) technique. The differences between the diameters of the GNPs that were calculated using this method and those that were measured using SEM were less than 5 nm. The RTW method has also been applied in the monitoring of the refractive index of the media surrounding the GNPs, and their dynamic acting within cells in real-time.