文献类型：期刊文献

英文题名：Correlation of particle properties with cytotoxicity and cellular uptake of hydroxyapatite nanoparticles in human gastric cancer cells

作者：Cui, Xinhui[1];Liang, Tong[2];Liu, Changsheng[1,2];Yuan, Yuan[2];Qian, Jiangchao[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2016

卷号：67

起止页码：453

外文期刊名：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20162302457347);WOS:【SCI-EXPANDED(收录号:WOS:000378952800051)】；

基金：We appreciate the financial support from the National Basic Research Program of China (973 Program, 2012CB933600), the National Natural Science Foundation of China (31271010), and Program for New Century Excellent Talents in University (NCET-11-0639). The authors are cordially thankful to Professor Yifan Han and Dr. Like Ouyang for the Raman measurements.

语种：英文

外文关键词：Hydroxyapatite nanoparticles; Particle properties; Cancer cell; Cytotoxicity; Uptake; Intracellular calcium concentration

摘要：Three types of hydroxyapatite nanoparticles (HAPNs) were synthesized employing a sonochemistry-assisted microwave method by changing microwave power (from 200 to 300 W) or using calcination treatment: 1200 (200 W, lyophilization), 1300 (300 W, lyophilization) and C200 (200 W, lyophilization & calcination). Their physiochemical properties were characterized and correlated with cytotoxicity to human gastric cancer cells (MGC80-3). The major differences among these HAPN preparations were their size and specific surface area, with the 1200 showing a smaller size and higher specific surface area. Although all HAPNs inhibited cell proliferation and induced apoptosis of cancer cells,1200 exhibited the greatest toxicity. All types of HAPNs were internalized through energy-dependent pathways, but the 1200 nanoparticles were more efficiently uptaken by MGC80-3 cells. Inhibitor studies with dynasore and methyl-beta-cyclodextrin suggested that caveolae-mediated endocytosis and, to a much lesser extent, clathrin-mediated endocytosis, were involved in cellular uptake of the various preparations, whereas the inhibition of endocytosis was more obvious for L200. Using fluorescein isothiocyanate-labeled HAPNs and laser-scanning confocal microscopy, we found that all forms of nanoparticles were present in the cytoplasm, and some L200 HAPNs were even found within nuclei. Treatment with all HAPN preparations led to the increase in the intracellular calcium level with the highest level detected for L200. (C) 2016 Elsevier B.V. All rights reserved.