文献类型：期刊文献

英文题名：Effects of divalent metal cations on lovastatin biosynthesis from Aspergillus terreus in chemically defined medium

作者：Jia, Zhihua[2];Zhang, Xiaoli[1];Zhao, Yaling[1];Cao, Xuejun[2]

机构：[1]NW Univ Xian, Coll Chem Engn, Xian 710069, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：25

期号：7

起止页码：1235

外文期刊名：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000267059000013)】；

基金：Financial support from the Key Research Program sponsored by Bureau of Education of Shaanxi Province in China (99JK08) is gratefully acknowledged.

语种：英文

外文关键词：Aspergillus terreus; Lovastatin; Monacolin J; Divalent metal ions; Biosynthesis

摘要：Effects of six divalent metal cations: Fe2+, Ca2+, Zn2+, Mg2+, Cu(2+)and Mn2+ on fungal cell growth and lovastatin biosynthesis were investigated by submerged cultivation of Aspergillus terreus in a modified chemically defined medium. The influences of different initial concentrations of the above six metal cations were also examined at 1, 2, and 5 mM, respectively. Cu2+ apparently inhibited the cell growth, but had no influence on biosynthesis of lovastatin. All of Fe2+, Ca2+, Zn2+, Mg2+ and Mn2+ promoted the cell growth and lovastatin biosynthesis in different extents. The highest biomass of 13.8 +/- A 0.5 g l(-1) and specific lovastatin titres of 49.2 +/- A 1.4 mg gDCW(-1) were obtained at the level of 2 and 5 mM in the presence of Zn2+, respectively. The values were improved double and 14.4-fold. Excess Zn2+ inhibited the cell growth, but enhanced lovastatin biosynthesis with an increment of 17.6 mg l(-1) per mM. The interactions of all metal cations slightly inhibited the lovastatin production comparing with the existence of Zn2+, Fe2+ and Mg2+ solely, yet remarkably improved the cell growth. These results suggest that the divalent metal ions Zn2+ or Fe2+ influence the production by regulating the action of key enzymes such as LovD or LovF in lovastatin biosynthesis.