文献类型：期刊文献

中文题名：四环素类抗生素降解菌的筛选及降解特性探究

英文题名：Screening and Degradation Characteristics of Tetracyclines Degrading Bacteria

作者：曹欢[1];黄泽昊[2];苏彩萍[2];任宇红[1]

机构：[1]华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海200237;[2]上海舒圣投资管理有限公司,上海201399

年份：2022

卷号：45

期号：12

起止页码：19

中文期刊名：环境科学与技术

外文期刊名：Environmental Science & Technology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

语种：中文

中文关键词：四环素;土霉素;强力霉素;生物降解;降解特性

外文关键词：tetracycline;oxytetracycline;doxycycline;biodegradable;degradation characteristics

摘要：利用微生物降解环境中的抗生素是一种绿色有效的方式。为了减轻环境中大量残留的四环素类抗生素(TCs)污染,文章从生物肥料样品中分离得到2株具有同时降解四环素(TC)和土霉素(OTC)能力的Lysinibacillus sp.菌株,分别命名为TO1和TO2;以及2株具有降解强力霉素(DC)能力的菌株,分别命名为D1(Providencia sp.)和D2(Proteus sp.)。在以TCs为唯一碳源的条件下,探究接种量、初始抗生素浓度、温度及p H对TCs降解率的影响,结果表明:TO1、TO2对OTC的生物降解率在第4天最高可达到30.15%和32.59%,总降解率分别为91.50%和92.94%。在第7天,TO1、TO2对TC的生物降解率最高可达到61.81%和59.20%,总降解率分别为88.66%和91.61%;且D1、D2对DC的生物降解率最高可达到48.21%和52.73%,总降解率分别为56.15%和60.22%。选取Bacillus subtilis和Escherichia coli作为毒性指示菌以评估TCs生物降解反应中的产物毒性,结果显示:生物降解产物的毒性显著低于水解产物,证明微生物降解抗生素对环境安全友好,具有实际应用前景。
Using microorganisms to degrade antibiotics in the environment is a green and effective way.In order to mitigate the contamination of large amounts of residual tetracycline antibiotics(TCs)in the environment,two strains of Lysinibacillus sp.with the ability to degrade both tetracycline(TC)and oxytetracycline(OTC),named TO1 and TO2,respectively,and two strains with the ability to degrade doxycycline(DC),named D1(Providencia sp.)and D2(Proteus sp.),respectively,were isolated from biofertilizer samples.The effects of inoculum,initial antibiotic concentration,temperature and pH on the degradation rate of TCs were investigated under the condition of using TCs as the only carbon source,the results were obtained:the biodegradation rates of TO1 and TO2 for OTC could reach up to 30.15%and 32.59%on the fourth day,and the total degradation rates were 91.50%and 92.94%,respectively.On the seventh day,the highest biodegradation rates of TO1 and TO2 to TC could reach 61.81%and 59.20%,and the total degradation rates were 88.66%and 91.61%,respectively;and the highest biodegradation rates of D1 and D2 to DC could reach 48.21%and 52.73%,and the total degradation rates were 56.15%and 60.22%,respectively.Bacillus subtilis and Escherichia coli were selected as toxicity indicator bacteria to assess the toxicity of products in the biodegradation reaction of TCs.The results showed that the toxicity of biodegradation products was significantly lower than that of hydrolysis products,which proved that microbial degradation of antibiotics is environmentally safe and friendly,and has practical application prospects.