文献类型：期刊文献

英文题名：Highly sensitive and fast responsive fluorescence turn-on chemodosimeter for Cu²⁺ and its application in live cell imaging

作者：Yu, Mengxiao[1,2];Shi, Mei[1,2];Chen, Zhigang[1,2];Li, Fuyou[1,2];Li, Xinxin[3];Gao, Yanhong[4];Xu, Jia[1,2];Yang, Hong[1,2];Zhou, Zhiguo[1,2];Yi, Tao[1,2];Huang, Chunhui[1,2]

机构：[1]Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China;[2]Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China;[3]E China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[4]Shanghai Jiao Tong Univ, Hosp Xinhua, Sch Med, Shanghai 200092, Peoples R China

年份：2008

卷号：14

期号：23

起止页码：6892

外文期刊名：CHEMISTRY-A EUROPEAN JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000258683600010)】；

基金：The authors are thankful for the financial support from National Natural Science Foundation of China (20490201, 20501006 and 20775017). National High Technology Program of China (2006 AA03Z318), NCET-06-0353, Shanghai Science and Technology Community (06QH14002). Huo Yingdong Education Foundation (10401-2) and Shanghai Leading academic Discipline Project (13108). We also thank Professor Cong-Jian Xu and Dr. Xiao-Yan Zhang for their helpful dicussion.

语种：英文

外文关键词：chemodosimeter; copper; fluorescent probes; imaging agents; rhodamine

摘要：A rhodamine B derivative 4 containing a highly electron-rich S atom has been synthesized as a fluorescence turn-on chemodosimeter for Cu2+. Following Cu2+-promoted ring-opening, redox and hydrolysis reactions, comparable amplifications of absorption and fluorescence signals were observed upon addition of Cu2+ this Suggests that chemodosimeter 4 effectively avoided the fluorescence quenching caused by the paramagnetic nature of Cu2+. Importantly, 4 can selectively recognize Cu2+ in aqueous media in the presence of other trace metal ions in organisms (Such as Fe3+ Fe2+, Cu+, Zn2+, Cr3+, Mn2+, Co2+, and Ni2+), abundant cellular cations (such as Na+, K+, Mg2+ and Ca2+), and the prevalent toxic metal ions in the environment (Such as Pb2+ and Cd2+) Will, high sensitivity (detection limit <= 10 ppb) and a rapid response time (<= 1 min). Moreover, by virtue of the chemodosimeter as fluorescent probe for Cu2+, confocal and two-photon microscopy experiments revealed a significant increase of intracellular Cu2+ concentration and the subcellular distribution of Cu2+. which was internalized into the living HeLa cells upon incubation in growth medium supplemented with 50 mu M CuCl2 for 20 h.