文献类型：期刊文献

英文题名：Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30

作者：Zhang, Jiajia[1];Wu, Chuan[1];Wang, Wei[1,2];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, POB 311,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：5

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513079743);WOS:【SCI-EXPANDED(收录号:WOS:000461106800040)】；

基金：This research was supported by the National Natural Science Foundation of China (No. C010302-31500066) and the Fundamental Research Funds for the Central Universities (No. 222201714053).

语种：英文

外文关键词：Enhanced transcriptional activator; Trichoderma reesei; Transcription factor; Cellulase; XYR1; ACE2; ACE1

摘要：Enhancing cellulase production in Trichoderma reesei is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in T reesei. In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16. T reesei transformants T-XYR1VP, T-ACE2VP, and T-ACE1VP showed improved cellulase and/or xylanase production. T-XYR1VP has a cellulase-free phenotype but with significantly elevated xylanase production. Xylanase I and xylanase II activities [U/(mg biomass)] increased by 51% and 80%, respectively, inT(XYR1VP) in comparison with parental strain RUT C30. The filter paper activity of T ACE2VP in the Avicel-based medium increased by 52% compared to that of RUT C30. In the Avicel-based medium, T-XYR1VP manifested an 80% increase in FPase activity and a 50% increase in xylanase activity as compared to those of RUT C30. Additionally, when pretreated corn stover was hydrolyzed, crude enzymes produced from T-XYR1VP yielded a greater glucose release than did the enzymes produced by parental strain RUT C30.