文献类型：期刊文献

英文题名：CRISPR/Cas9-mediated targeted mutagenesis of bZIP2 in Salvia miltiorrhiza leads to promoted phenolic acid biosynthesis

作者：Shi, Min[1,2];Du, Zhiyan[3];Hua, Qiang[1];Kai, Guoyin[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Chinese Med Univ, Coll Pharm, Lab Med Plant Biotechnol, Hangzhou 310053, Zhejiang, Peoples R China;[3]Univ Hawaii Manoa, Dept Mol Biosci & Bioengn, 1955 East West Rd 218, Honolulu, HI 96822 USA

年份：2021

卷号：167

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20211810306695);WOS:【SCI-EXPANDED(收录号:WOS:000652531900009)】；

基金：This work was funded by the National Natural Science Fund of China (82073963, 81522049, 31571735, 31270007), Shanghai Science and Technology Committee Project (17JC1404300, 15430502700), Zhejiang Provincial Ten-thousand Program for Leading Talents of Science and Technology Innovation (2018R52050), Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents, the "Dawn" Program of Shanghai Education Commission (16SG38) and Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University (Traditional Chinese Pharmacology), Zhejiang Chinese Medical University (No. ZYAOXZD2019004).

语种：英文

外文关键词：Salvia miltiorrhiza; bZIP2; Phenolic acids; Abscisic acid; Transcriptional regulation

摘要：Phenolic acids comprise the major bioactive compounds in Salvia miltiorrhiza, which can be increased by abscisic acid (ABA) elicitation. However, the underlying regulation mechanism is poorly understood. Here, the ABAinduced transcriptome revealed that bZIP2 was significantly induced, which expressed highly in roots. Bioinformatic analysis revealed that bZIP2 belong to the subgroup A and contained a bZIP domain together with four conservative regions (C1, C2, C3, and C4). Phenolic acid content was increased in bZIP2 knock-out lines medicated by CRISPR/Cas9 technology. In contrast, overexpressing lines produced less phenolic acids by downregulating the PAL gene. Biochemistry assays showed that bZIP2 binds to the ABRE2 element in the promoter region of the PAL gene and suppresses its expression. Besides, bZIP2 physically interacted with SnRK2.3, SnRK2.4, SnRK2.6, and SnRK2.10. Overall, these results demonstrated that bZIP2 is a negative regulator in phenolic acid biosynthesis, which provides new insights for the metabolic engineering of phenolic acids.